SERS detection of proteins on micropatterned protein-mediated sandwich substrates

Authors

  • Weidong Ruan,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    2. Engineering Laboratory for Modern Analytical Techniques, State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, China
    Search for more papers by this author
  • Wei Ji,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Xiangxin Xue,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Yang Cui,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Lei Chen,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Tieli Zhou,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Li Niu,

    Corresponding author
    1. Engineering Laboratory for Modern Analytical Techniques, State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, China
    • Engineering Laboratory for Modern Analytical Techniques, State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun 130022, China.
    Search for more papers by this author
  • Xiao Li,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Junhu Zhang,

    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    Search for more papers by this author
  • Bing Zhao

    Corresponding author
    1. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
    • State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China.
    Search for more papers by this author

Abstract

One of the greatest challenges in developing protein chips is the detection of trace amounts of proteins on their surfaces. Traditionally employed techniques, such as optical microscopy and fluorescence, are effective and widely used, but it is sometimes hard to obtain fingerprint signals of biomolecules. In this paper, we use surface-enhanced Raman scattering (SERS) spectroscopy as a platform for protein detection. Micropatterned protein-mediated Au/Ag sandwich structures were employed as the detecting objects. Two types of proteins, pure hemoprotein and immunocomplex, were used as the media. Au/Ag layers were used as the SERS substrates. The resulting spectra showed good sensitivity and resolution. It indicates that SERS is a powerful tool in protein detection and has great potential for application in protein chips. Copyright © 2011 John Wiley & Sons, Ltd.

Ancillary