Drop-coating deposition Raman spectroscopy of liposomes
Version of Record online: 28 MAR 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 42, Issue 8, pages 1606–1610, August 2011
How to Cite
Kočišová, E. and Procházka, M. (2011), Drop-coating deposition Raman spectroscopy of liposomes. J. Raman Spectrosc., 42: 1606–1610. doi: 10.1002/jrs.2915
- Issue online: 18 AUG 2011
- Version of Record online: 28 MAR 2011
- Manuscript Accepted: 15 JAN 2011
- Manuscript Received: 20 DEC 2010
- drop-coating deposition Raman;
Drop-coating deposition Raman (DCDR) spectroscopy was tested as a potential technique for studying liposomes at very low sample concentrations. We used model liposomes prepared either from 1,2-distearoyl-sn-glycero-3-phospocholine or from soybean asolectin, which is composed of various lipids and thus represents a good model of natural membranes. In both cases, deposited samples formed a dried drop with a circular shape with a ring of concentrated liposomes at the edge. Spectral mapping showed that maximum Raman intensity originated from the inner part of the edge ring, while Raman signal gradually decreased in both radial directions. The Raman spectra exhibited excellent reproducibility of spectral characteristics at different locations in the drop, indicating similar conformation and ordering of hydrocarbon lipid chains in the sample. Our results suggest that DCDR spectroscopy can be used for studying lipids in situ, and sensitivity of this technique is at least two orders of magnitude higher than that of conventional Raman microscopy. Copyright © 2011 John Wiley & Sons, Ltd.