Leucine pools in Escherichia coli biofilm discovered by Raman imaging
Article first published online: 6 APR 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 42, Issue 11, pages 1913–1915, 15 November 2011
How to Cite
Noothalapati Venkata, H. N., Nomura, N. and Shigeto, S. (2011), Leucine pools in Escherichia coli biofilm discovered by Raman imaging. J. Raman Spectrosc., 42: 1913–1915. doi: 10.1002/jrs.2952
- Issue published online: 10 NOV 2011
- Article first published online: 6 APR 2011
- Manuscript Accepted: 21 FEB 2011
- Manuscript Received: 3 JAN 2011
- ‘Aiming for the Top University Plan’ of National Chiao Tung University and the Ministry of Education (Taiwan)
- National Science Council of Taiwan. Grant Number: NSC97-2113-M-009-002-MY2
- Raman microspectroscopy;
- label-free imaging;
In structured communities of bacteria known as biofilms, a variety of biomolecules have been shown to play a unique role as signals and/or regulators in biofilm formation. Here, we report that high levels of the amino acid leucine (leucine pool) were detected, for the first time, within microcolonies in a 30-h-old Escherichia coli biofilm by Raman imaging. Localization of leucine revealed by multifrequency Raman images indicates leucine accumulation during the early stage of the E. coli biofilm formation, which may have resulted from physiological environment-specific metabolic adaptation. We demonstrate that our label-free Raman imaging method provides a useful platform for directly identifying still unknown natural products produced in biofilms as well as for visualizing heterogeneous distributions of biofilm constituents in situ. Copyright © 2011 John Wiley & Sons, Ltd.