Investigation of adhesive–dentin interfaces using Raman microspectroscopy and small angle X-ray scattering
Article first published online: 28 JUN 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 43, Issue 1, pages 6–15, January 2012
How to Cite
Bayarı, S. H., Krafft, C., İde, S., Popp, J., Guven, G., Cehreli, Z. C. and Soylu, E. H. (2012), Investigation of adhesive–dentin interfaces using Raman microspectroscopy and small angle X-ray scattering. J. Raman Spectrosc., 43: 6–15. doi: 10.1002/jrs.3008
- Issue published online: 27 JAN 2012
- Article first published online: 28 JUN 2011
- Manuscript Accepted: 4 MAY 2011
- Manuscript Received: 19 JAN 2011
- dental adhesives;
- dentin-adhesive interface;
- Raman microspectroscopy;
Human dentin specimens were treated with two different etch-and-rinse adhesives, Single Bond 2 (SB2) and Prime & Bond NT (PBNT), and two composite resins, TPH and P60. Cross-sectional samples, approximately 1 mm thick, were analyzed with Raman line mapping and imaging across the dentin–adhesive–composite interface. The integrated intensities of selected bands associated with adhesive, organic material, composite and hydroxyapatite of dentin were calculated to determine the distribution of adhesive infiltration into demineralized dentin. The results were compared with the enamel-adhesive composite interface. The demineralized zone was smaller in the enamel-adhesive interface than in the dentin–adhesive interface. The region of collagen-adhesive crosslinking was wider in the PBNT adhesive than in the SB2 adhesive. However, a gap at the dentin–PBNT composite interface, which was not observed at the dentin–SB2 composite interface, might compromise the dentin–restoration bond. K-means cluster analysis of the Raman images confirmed the findings. The ultrastructure of the dentin–resin interface was studied using scanning electron microscopy. Small-angle X-ray scattering was also applied to reveal and quantify fine-scale structural features. SB2 adhesive was found to diffuse more into demineralized dentin along with greater nanosized aggregations in the hybrid layer. Copyright © 2011 John Wiley & Sons, Ltd.