SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide–antibody assays

Authors

  • Alessandro Virga,

    1. Materials Science and Chemical Engineering Department, Politecnico of Torino, Torino, Italy
    2. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
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  • Paola Rivolo,

    1. Materials Science and Chemical Engineering Department, Politecnico of Torino, Torino, Italy
    2. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
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  • Emiliano Descrovi,

    1. Materials Science and Chemical Engineering Department, Politecnico of Torino, Torino, Italy
    2. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
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  • Alessandro Chiolerio,

    1. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
    2. Physics Department, Politecnico of Torino, Torino, Italy
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  • Gabriella Digregorio,

    1. Materials Science and Chemical Engineering Department, Politecnico of Torino, Torino, Italy
    2. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
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  • Francesca Frascella,

    1. Materials Science and Chemical Engineering Department, Politecnico of Torino, Torino, Italy
    2. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
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  • Marco Soster,

    1. Oncological Sciences Department, University of Torino, School of Medicine, Candiolo, Italy
    2. Laboratory of Tumor Microenvironment, Institute for Cancer Research and Treatment, Candiolo, Italy
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  • Federico Bussolino,

    1. Oncological Sciences Department, University of Torino, School of Medicine, Candiolo, Italy
    2. Laboratoryof Vascular Oncology, Institute for Cancer Research and Treatment, Candiolo, Italy
    3. APAvadis Biotechnologies srl, BioIndustry Park Silvano Fumero, Colleretto Giacosa, Italy
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  • Serena Marchiò,

    1. Oncological Sciences Department, University of Torino, School of Medicine, Candiolo, Italy
    2. Laboratory of Tumor Microenvironment, Institute for Cancer Research and Treatment, Candiolo, Italy
    3. APAvadis Biotechnologies srl, BioIndustry Park Silvano Fumero, Colleretto Giacosa, Italy
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  • Francesco Geobaldo,

    1. Materials Science and Chemical Engineering Department, Politecnico of Torino, Torino, Italy
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  • Fabrizio Giorgis

    Corresponding author
    1. Materials and Microsystems Laboratory χLab, Politecnico of Torino, Torino, Italy
    • Physics Department, Politecnico of Torino, Torino, Italy
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Fabrizio Giorgis, Physics Department, Politecnico of Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy. E-mail: fabrizio.giorgis@polito.it

Abstract

Ag nanoparticles synthesized on porous silicon samples were studied and applied as substrates for surface-enhanced Raman scattering (SERS). The metallic nanostructures prepared by immersion plating were characterized by UV–Vis reflectance spectroscopy and scanning electron microscopy. SERS activity of the substrates was tested using Cyanine dye 1,3,3,1′,3′,3′-esamethyl-5,5′-dimethoxyindodicarbocyanine iodide (Cy5-OCH3) as a probe molecule. The Raman spectra obtained for different excitation wavelengths indicate amplifications ascribed to plasmonic resonances with an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays. Copyright © 2011 John Wiley & Sons, Ltd.

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