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Spacer-free SERRS spectra of unperturbed porphyrin detected at 100 fM concentration in Ag hydrosols prepared by modified Tollens method

Authors

  • Karolína Šišková,

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    • Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry Faculty of Science, Palacky University, Olomouc, Czech Republic
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  • Ondřej Bečička,

    1. Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry Faculty of Science, Palacky University, Olomouc, Czech Republic
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  • Vlastimil Mašek,

    1. Department of Pharmacology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
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  • Klára Šafářová,

    1. Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry Faculty of Science, Palacky University, Olomouc, Czech Republic
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  • Radek Zbořil

    1. Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry Faculty of Science, Palacky University, Olomouc, Czech Republic
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Karolína Šišková, Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacky University, 17. listopadu 12, 77146 Olomouc, Czech Republic.

E-mail: karolina.siskova@upol.cz

Abstract

Surface-enhanced resonance Raman scattering (SERRS) spectroscopy is a powerful tool for the selective and sensitive detection of porphyrins. We report a repeatable detection of unperturbed free base 5,10,15,20-tetrakis(1-methyl-4-pyridyl)21H,23H-porphine in concentrations as low as 1 × 10–13 M in the final system when Ag nanoparticles prepared by modified Tollens method are employed as Raman scattering amplifiers, i.e. without any further post-modifications of their surface. Most probably the residues of sugar oxidation present on Ag nanoparticle surface are responsible for the effective protection of 5,10,15,20-tetrakis(1-methyl-4-pyridyl)21H,23H-porphine molecules against the metalation by silver. Copyright © 2011 John Wiley & Sons, Ltd.

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