Current address: Argonne National Laboratory, Materials Science Division, Argonne, IL 60439, USA.
Nanoprobes for intracellular and single cell surface-enhanced Raman spectroscopy (SERS)
Version of Record online: 13 MAR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 43, Issue 7, pages 817–827, July 2012
How to Cite
Vitol, E. A., Orynbayeva, Z., Friedman, G. and Gogotsi, Y. (2012), Nanoprobes for intracellular and single cell surface-enhanced Raman spectroscopy (SERS). J. Raman Spectrosc., 43: 817–827. doi: 10.1002/jrs.3100
- Issue online: 19 JUL 2012
- Version of Record online: 13 MAR 2012
- Manuscript Accepted: 5 OCT 2011
- Manuscript Revised: 3 OCT 2011
- Manuscript Received: 10 MAY 2011
- carbon nanotubes
Surface-enhanced Raman spectroscopy (SERS) is a promising and powerful label free technique for high resolution analysis of single cells. For intracellular analysis, there is a need for SERS-active nanoprobes that are minimally invasive to cells, do not affect cell viability, and provide reproducible signals. This work reviews the state-of-the-art tools currently available for intracellular SERS. Various types of SERS probes are considered, including colloidal gold and silver nanoparticles, metallized optical fibers, and tip-enhanced Raman probes. We also discuss recently developed SERS-active nanopipettes implemented on the basis of pulled glass microcapillaries. Finally, the critical aspects of selecting an optimal SERS nanoprobe for single-cell analysis depending on a particular application are summarized. Copyright © 2012 John Wiley & Sons, Ltd.