Investigation of organelle-specific intracellular water structures with Raman microspectroscopy
Article first published online: 8 NOV 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 44, Issue 2, pages 167–169, February 2013
How to Cite
Tiwari, S., Ando, M. and Hamaguchi, H.-o. (2013), Investigation of organelle-specific intracellular water structures with Raman microspectroscopy. J. Raman Spectrosc., 44: 167–169. doi: 10.1002/jrs.4165
- Issue published online: 24 JAN 2013
- Article first published online: 8 NOV 2012
- Manuscript Accepted: 13 JUL 2012
- Manuscript Revised: 23 JUN 2012
- Manuscript Received: 30 MAR 2012
- Raman microspectroscopy;
- living cells;
- multivariate analysis
The study of intracellular water in living cells is a challenge of fundamental importance. While the critical roles of water in maintaining and propagating life have been widely recognized and accepted, our understanding of water/biomolecule interactions is surprisingly limited. Using Raman microspectroscopic imaging of a living yeast cell followed by a multivariate analysis in form of the nonnegative matrix factorization method, we successfully resolve organelle-specific water structures. The intensities and the band profiles of the segregated water OH stretch spectra yield important and otherwise unobtainable information on the extensive effect of the water/biomolecule interactions in a given organelle on the hydrogen-bonding network of water molecules. Copyright © 2012 John Wiley & Sons, Ltd.