SERS as a valuable tool for detection and treatment follow-up of fungal infection in mice lungs: use of Amphotericin B and its nanoencapsulation onto magnetic nanoparticles
Article first published online: 25 FEB 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 44, Issue 5, pages 695–702, May 2013
How to Cite
Santos, C. M. B., da Silva, S. W., Saldanha, C. A., Santos, M. F. M. A., Garcia, M. P., Azevedo, R. B. and Morais, P. C. (2013), SERS as a valuable tool for detection and treatment follow-up of fungal infection in mice lungs: use of Amphotericin B and its nanoencapsulation onto magnetic nanoparticles. J. Raman Spectrosc., 44: 695–702. doi: 10.1002/jrs.4259
- Issue published online: 10 MAY 2013
- Article first published online: 25 FEB 2013
- Manuscript Accepted: 7 JAN 2013
- Manuscript Revised: 20 DEC 2012
- Manuscript Received: 30 OCT 2012
- Amphotericin B;
- drug delivery;
- fungal infection
This study reports on the use of surface-enhanced Raman scattering (SERS) detection and follow-up treatment of Paracoccidioides brasiliensis (Pb) infection in lung's mice. We also reports on the introduction of a new drug carrier system [nanoparticle-Amphotericin B (NP-AmB)], comprising magnetic NP surface functionalized with AmB, and its use in the treatment of infected and non-infected mice. SERS was successfully used to monitor the efficacy of the mice's treatment using the new NP-AmB, while free AmB (F-AmB), considering the current drug of choice for treatment of Pb infection, was also used and taken as reference for the treatment. We found SERS provides a robust platform to discriminate infected lung tissues from non-infected ones based on fingerprints assessed via SERS spectra and focused on the redox state of heme groups present in the collected biological material. Finally, SERS data reported in this study indicated that the new NP-AmB formulation provides similar clinical response as the F-AmB, although incorporating 40% lower content of AmB and administered in a time interval schedule (every 72 h) three times longer than F-AmB (every 24 h). Copyright © 2013 John Wiley & Sons, Ltd.