Surface-enhanced Raman scattering assessment of DNA from leaf tissues adsorbed on silver colloidal nanoparticles
Article first published online: 9 APR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 44, Issue 6, pages 817–822, June 2013
How to Cite
Muntean, C. M., Leopold, N., Halmagyi, A. and Valimareanu, S. (2013), Surface-enhanced Raman scattering assessment of DNA from leaf tissues adsorbed on silver colloidal nanoparticles. J. Raman Spectrosc., 44: 817–822. doi: 10.1002/jrs.4265
- Issue published online: 5 JUN 2013
- Article first published online: 9 APR 2013
- Manuscript Accepted: 16 JAN 2013
- Manuscript Revised: 15 JAN 2013
- Manuscript Received: 2 OCT 2012
- surface-enhanced Raman scattering (SERS);
- leaf tissue;
- silver colloidal nanoparticles
In this work, the surface-enhanced Raman scattering (SERS) spectra of seven genomic DNAs from leaves of chrysanthemum (Dendranthema grandiflora Ramat.), common sundew (Drosera rotundifolia L.), edelweiss (Leontopodium alpinum Cass), Epilobium hirsutum L., Hypericum richeri ssp. transsilvanicum (Čelak) Ciocârlan, rose (Rosa x hybrida L.) and redwood (Sequoia sempervirens D. Don. Endl.), respectively, have been analyzed in the wavenumber range 200–1800 cm−1. The surface-enhanced Raman vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. A high molecular structural information content can be found in the SERS spectra of these DNAs from leaf tissues. Based on this work, specific plant DNA–ligand interactions or accurate local structure of DNA might be further investigated using surface-enhanced Raman spectroscopy. Besides, this study will generate information which is valuable in the development of label-free DNA detection for chemical probing in living cell. Copyright © 2013 John Wiley & Sons, Ltd.