Application of silver nanoparticle-based SERS spectroscopy for DNA analysis in radiated nasopharyngeal carcinoma cells

Authors

  • Lin Ou,

    1. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China
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    • Lin Ou and Yang Chen are co-first authors; they contributed equally to this work.

  • Yang Chen,

    Corresponding author
    • Zhicheng College, Fuzhou University, Fuzhou, China
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    • Lin Ou and Yang Chen are co-first authors; they contributed equally to this work.

  • Ying Su,

    1. Laboratory of Radiobiology, Fujian Provincial Tumor Hospital, Fuzhou, China
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  • Yangwen Huang,

    1. Key Laboratory of Instrumentation Science and Dynamic Measurement (North University of China), Ministry of Education, North University of China, Taiyuan, China
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  • Rong Chen,

    1. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China
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  • Jinping Lei

    1. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou, China
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Correspondence to: Yang Chen: Zhicheng College, Fuzhou University, Fuzhou, China

Email: chzhy85@163.com.

Abstract

This work aims to explore the application of silver nanoparticle-based surface-enhanced Raman scattering (SERS) for nasopharyngeal carcinoma cell line CNE2's DNA analysis after X-ray radiation. The cells are separated into control group and radiated groups with different dose of 6, 10, 15 and 20 Gy. The results show that after radiation (6, 10, 15 and 20 Gy), the DNA of radiated CNE2 have changed after 72 h of cell incubation. Principal components analysis is employed for significant differences and the DNA extracted after 72 h of incubation show significant divisions from control group. Moreover, a classifier based on support vector machines shows high classification accuracy between DNA extracted after 72 h of incubation and control group. In conclusion, this study first reveals SERS characteristics of CNE2's DNA under different dose of X-ray radiation, and the final results may do favor to make known the mechanism of X-ray radiation interacting with tumor. Copyright © 2013 John Wiley & Sons, Ltd.

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