This article is from the GISR part of the joint special issue on the European Conference on Nonlinear Optical Spectroscopy (ECONOS 2012) with Guest Editors Johannes Kiefer and Peter Radi and the II Italian Conference of the National Group of Raman Spectroscopy and Non-Linear Effects (GISR 2012) with Guest Editor Maria Grazia Giorgini.
Vibrational characterization and surface-enhanced Raman scattering detection of probenecid doping drug
Article first published online: 5 APR 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Special Issue: Joint special issue on the European Conference on Nonlinear Optical Spectroscopy (ECONOS 2012) and the II Italian Conference of the National Group of Raman Spectroscopy and Non-Linear Effects (GISR 2012)
Volume 44, Issue 10, pages 1422–1427, October 2013
How to Cite
Izquierdo-Lorenzo, I., García-Ramos, J. V. and Sanchez-Cortes, S. (2013), Vibrational characterization and surface-enhanced Raman scattering detection of probenecid doping drug. J. Raman Spectrosc., 44: 1422–1427. doi: 10.1002/jrs.4284
- Issue published online: 22 OCT 2013
- Article first published online: 5 APR 2013
- Manuscript Accepted: 22 JAN 2013
- Manuscript Revised: 22 DEC 2012
- Manuscript Received: 9 NOV 2012
- Spanish Ministerio de Ciencia e Innovación. Grant Number: FIS2010-15405
- Comunidad de Madrid through the MICROSERES II network. Grant Number: S2009/TIC-1476
Probenecid (PB), a drug employed to reduce the excretion of other drugs in urine and also employed as masking agent in sport doping, is characterized for the first time in this work by vibrational spectroscopy. Previously, FTIR and FT-Raman spectra were assigned on the basis of density functional theory calculations. Afterwards, a detection protocol of PB was developed on the basis of surface-enhanced Raman scattering (SERS) spectroscopy. To accomplish this, the experimental conditions of SERS measurement were previously optimized by varying parameters such as the metal, its reduction protocol to fabricate the nanoparticles and the pH. The adsorption isotherms of PB on the metal nanoparticles were obtained, and the adsorption mechanism of this drug on Ag nanoparticles was deduced from the analysis of SERS spectra. Finally, a quantitative SERS study was carried out on the basis of the calibration curve deduced from the adsorption isotherms. The results shown in this study demonstrate that PB can be detected by SERS at concentrations as low as 1.2 µg/ml. Copyright © 2013 John Wiley & Sons, Ltd.