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Micro-Raman spectroscopy identifies crocidolite and erionite fibers in tissue sections


  • This article is from the GISR part of the joint special issue on the European Conference on Nonlinear Optical Spectroscopy (ECONOS 2012) with Guest Editors Johannes Kiefer and Peter Radi and the II Italian Conference of the National Group of Raman Spectroscopy and Non-Linear Effects (GISR 2012) with Guest Editor Maria Grazia Giorgini.


Exposure to asbestos or erionite is associated with malignant mesothelioma (MM) and other diseases. Micro-Raman spectroscopy and Variable Pressure Scanning Electron Microscopy/Energy Dispersive Spectroscopy were used to identify mineral fibers in histological sections of various tissues taken from mice injected with crocidolite asbestos and erionite fibers. Routine diagnostic histopathological slides of biopsies from erionite-exposed MM patients from Turkey were also analyzed. We show here that micro-Raman spectroscopy easily permits the recording of distinct Raman patterns of both crocidolite and erionite fibers, in both murine tissues and in human biopsies. The described findings provide a new powerful and non-destructive tool to determine the type of fiber exposure in patients with MM and other mineral fiber-associated diseases. Copyright © 2013 John Wiley & Sons, Ltd.