This article was published online on 2 September 2013. This notice was included in the online version to indicate that the Acknowledgements have been modified [19 September 2013].
Improved methods for fluorescence background subtraction from Raman spectra†
Article first published online: 2 SEP 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 44, Issue 11, pages 1587–1595, November 2013
How to Cite
Cadusch, P. J., Hlaing, M. M., Wade, S. A., McArthur, S. L. and Stoddart, P. R. (2013), Improved methods for fluorescence background subtraction from Raman spectra. J. Raman Spectrosc., 44: 1587–1595. doi: 10.1002/jrs.4371
- Issue published online: 8 NOV 2013
- Article first published online: 2 SEP 2013
- Manuscript Accepted: 27 JUL 2013
- Manuscript Revised: 13 JUN 2013
- Manuscript Received: 16 OCT 2012
- fluorescence background;
- background subtraction;
- adaptive-weight polynomial fit;
- adaptive-weight penalized least squares
Raman spectroscopy has attracted interest as a non-invasive optical technique to study the composition and structure of a wide range of materials at the microscopic level. The intrinsic fluorescence background can be orders of magnitude stronger than the Raman scattering, and so, background removal is one of the foremost challenges for quantitative analysis of Raman spectra in many samples. A range of methods anchored in instrumental and computational programming approaches have been proposed for removing fluorescence background signals. An enhanced adaptive weighting scheme for automated fluorescence removal is reported, applicable to both polynomial fitting and penalized least squares approaches. Analysis of the background fitting results for ensembles of simulated spectra suggests that the method is robust and reliable and can significantly improve the background fit over the range of signal, shot noise and background parameters tested, while reducing the subjective nature of the process. The method was also illustrated by application to experimental data generated from aqueous solutions of bulk protein fibrinogen mixed with dextran. Copyright © 2013 John Wiley & Sons, Ltd.