Optimising image quality in 2D and 3D confocal Raman mapping
Version of Record online: 18 DEC 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 45, Issue 1, pages 133–138, January 2014
How to Cite
Everall, N. (2014), Optimising image quality in 2D and 3D confocal Raman mapping. J. Raman Spectrosc., 45: 133–138. doi: 10.1002/jrs.4430
- Issue online: 16 JAN 2014
- Version of Record online: 18 DEC 2013
- Manuscript Accepted: 21 NOV 2013
- Manuscript Revised: 1 NOV 2013
- Manuscript Received: 28 MAY 2013
- Confocal Raman microscopy;
- 3D imaging;
- oil immersion
This article compares the quality of Raman images obtained using metallurgical and oil immersion objectives to map complex structures in two and three dimensions. While the performance of these objectives for depth profiling planar structures has been discussed at length in the literature, the same comparison has not yet been made for 3D mapping of complex objects, where additional complications are introduced by non-planar geometries. Studying samples with increasing complexity shows that the oil immersion objective is strongly preferred because it yields brighter images with better contrast, and eliminates some new and confusing artefacts that do not arise with simple planar objects. Copyright © 2013 John Wiley & Sons, Ltd.