Outdoor use of mobile Raman spectrometers to study the solutions and ices of amino acids
Article first published online: 8 JAN 2014
Copyright © 2014 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 45, Issue 2, pages 179–187, February 2014
How to Cite
Culka, A., Jehlička, J. and Čapoun, T. (2014), Outdoor use of mobile Raman spectrometers to study the solutions and ices of amino acids. J. Raman Spectrosc., 45: 179–187. doi: 10.1002/jrs.4432
- Issue published online: 6 FEB 2014
- Article first published online: 8 JAN 2014
- Manuscript Accepted: 28 NOV 2013
- Manuscript Revised: 27 NOV 2013
- Manuscript Received: 27 MAY 2013
- Grant Agency of the Czech Republic. Grant Numbers: P210/10/0467, MSM0021620855
- Ministry of Education of the Czech Republic
- amino acids;
Selected amino acids (glycine, alanine, and threonine) were analysed in the form of water solutions, mixtures of these amino acids in solution, as well as their respective ices using two hand-held instruments under outdoor and winter mountain conditions. The hand-held instruments featured 785 and 532 nm excitations; the latter being one of the first using this wavelength on the market. The changes to the Raman spectra of the solutions and ices are discussed in relationship to the degree of ambiguity of the detection of the amino acids within the mixtures. The hand-held instruments were able to detect two amino acids out of the three in solution. The results showed that the hand-held instruments provide sufficient quality spectra when analysing ices prepared from the solutions; however, an unambiguous detection of these amino acids within mixtures still remains a challenge. Copyright © 2014 John Wiley & Sons, Ltd.