Cancer gene detection using surface-enhanced Raman scattering (SERS)
Version of Record online: 10 JUL 2002
Copyright © 2002 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Special Issue: Medical Applications of Raman Spectroscopy
Volume 33, Issue 7, pages 511–516, July 2002
How to Cite
Vo-Dinh, T., Allain, L. R. and Stokes, D. L. (2002), Cancer gene detection using surface-enhanced Raman scattering (SERS). J. Raman Spectrosc., 33: 511–516. doi: 10.1002/jrs.883
- Issue online: 10 JUL 2002
- Version of Record online: 10 JUL 2002
- Manuscript Accepted: 13 MAR 2002
- Manuscript Received: 12 OCT 2001
- Office of Biological and Environmental Research, US Department of Energy. Grant Number: DE-AC05-960R22464.
- Lockheed Martin Energy Research Corporation.
- Federal Bureau of Investigation. Grant Number: 2051-II18-Y1.
- ORNL Laboratory Directed Research and Development Program (Advanced Nanosystems).
We describe the development of a surface-enhanced Raman scattering (SERS) method for cancer gene detection. The technology uses DNA gene probes based on SERS labels for gene detection. The detection method uses nanostructured metallic substrates as SERS-active platforms. The surface-enhanced Raman gene (SERGen) probes can be used to detect DNA targets via hybridization to DNA sequences complementary to these probes. The probes do not require the use of radioactive labels and have great potential to provide both sensitivity and selectivity. The usefulness of the SERGen approach and its applications in cancer gene diagnostics (e.g. BRCA1 breast cancer gene and BAX gene) is discussed. Copyright © 2002 John Wiley & Sons, Ltd.