Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy
Version of Record online: 17 DEC 2002
Copyright © 2002 John Wiley & Sons, Ltd.
Journal of Raman Spectroscopy
Volume 34, Issue 1, pages 1–3, January 2003
How to Cite
Huang, Y.-S., Karashima, T., Yamamoto, M. and Hamaguchi, H.-o. (2003), Molecular-level pursuit of yeast mitosis by time- and space-resolved Raman spectroscopy. J. Raman Spectrosc., 34: 1–3. doi: 10.1002/jrs.960
- Issue online: 17 DEC 2002
- Version of Record online: 17 DEC 2002
- Manuscript Accepted: 27 OCT 2002
- Manuscript Received: 25 OCT 2002
- time- and space-resolved Raman spectroscopy;
- living cell;
- cell division;
- fission yeast
The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time- and space-resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi-channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time-dependent changes of major molecular components during the cell cycle were elucidated. Copyright © 2002 John Wiley & Sons, Ltd.