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Keywords:

  • time- and space-resolved Raman spectroscopy;
  • living cell;
  • mitosis;
  • cell division;
  • fission yeast

Abstract

The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time- and space-resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi-channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time-dependent changes of major molecular components during the cell cycle were elucidated. Copyright © 2002 John Wiley & Sons, Ltd.