In vitro analysis of immersed human tissues by Raman microspectroscopy (pages 888–896)
F. Bonnier, A. Mehmood, P. Knief, A. D. Meade, W. Hornebeck, H. Lambkin, K. Flynn, V. McDonagh, C. Healy, T. C. Lee, F. M. Lyng and H. J. Byrne
Version of Record online: 4 NOV 2010 | DOI: 10.1002/jrs.2825
This study demonstrates that by measuring in immersion, Raman microscopy of tissue sections is greatly improved in terms of increased signal and signal to noise as well as reduced background. Data preprocessing is thus greatly simplified and the results significantly more reliable. The effect is understood as a significant reduction of Mie scattering of both the source laser and the Raman signal as a result of improved phase matching between the water/tissue interface compared to an air/water interface.