Imaging cortical vasculature with stimulated Raman scattering and two-photon photothermal lensing microscopy (pages 668–674)
Julian Moger, Natalie. L. Garrett, David Begley, Larisa Mihoreanu, Aikaterini Lalatsa, Maria Victoria Lozano, Mariarosa Mazza, Andreas Schatzlein and Ijeoma Uchegbu
Version of Record online: 26 APR 2012 | DOI: 10.1002/jrs.3156
The ability to map microvascular morphology and hemodynamic parameters, such as blood volume, is desirable for many biomedical studies and will lead to a deeper understanding of the mechanisms of angiogenesis and vascular disease. We present a novel approach that combines stimulated Raman scattering and two-photon photothermal lensing to provide simultaneous visualisation of cortical microvasular morphology and surrounding cellular structures. Volumetric analysis allows a direct quantification of local haematocrit volume rather than relying upon average plasma volume-to-haematocrit ratios.