Journal of Raman Spectroscopy

Cover image for Vol. 44 Issue 9

September 2013

Volume 44, Issue 9

Pages 1205–1316

  1. Research articles

    1. Top of page
    2. Research articles
    1. Confocal Raman microspectroscopy discriminates live human metastatic melanoma and skin fibroblast cells (pages 1205–1216)

      Andrew C. Terentis, Sara A. Fox, Samantha J. Friedman and Emily S. Spencer

      Version of Record online: 15 AUG 2013 | DOI: 10.1002/jrs.4363

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      Confocal Raman microspectroscopy was employed to study three distinct intracellular regions – cytoplasm, nucleoplasm, and nucleolus – within human metastatic melanoma (SK) and skin fibroblast (BJ) cells. Principal components analysis/linear discriminant analysis was 93-98% successful in discriminating BJ from SK cells, with higher RNA identified in the nucleoli of BJ cells and higher lipids and collagen identified in the cytoplasm of SK cells.

    2. Raman spectroscopy can discriminate distinct glioma subtypes as defined by RNA expression profiling (pages 1217–1221)

      Jan-Willem Jachtenberg, Tom Bakker Schut, Pim French, Max Kros, Martine Lamfers and Sieger Leenstra

      Version of Record online: 16 AUG 2013 | DOI: 10.1002/jrs.4350

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      In the field of personalized medicine, there is a great need to identify different subtypes of tumors. Present exploring techniques on DNA, RNA, or proteomics level are devious and expensive. In the present study, we show that Raman spectroscopy enables researchers to identify subtypes of tumors as defined by the before mentioned techniques.

    3. Calcification of aortic human valves studied in situ by Raman microimaging: following mineralization from small grains to big deposits (pages 1222–1229)

      Marta Pilarczyk, Krzysztof Czamara, Malgorzata Baranska, Joanna Natorska, Przemysław Kapusta, Anetta Undas and Agnieszka Kaczor

      Version of Record online: 15 AUG 2013 | DOI: 10.1002/jrs.4352

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      Raman microimaging has been used to evaluate formation and progression of calcification in situ in human stenotic aortic valves. The capability of the method to visualize distribution of the calcified deposits resulted in structural characterization of mineralization in the various phases of development.

    4. Raman comparison of skin dermis of different ages: focus on spectral markers of collagen hydration (pages 1230–1237)

      The Thuong Nguyen, Teddy Happillon, Jezabel Feru, Sylvie Brassart-Passco, Jean-François Angiboust, Michel Manfait and Olivier Piot

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/jrs.4355

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      Raman vibrations specific of collagen/water interactions were identified as potential photonic markers of skin collagen chronological aging.

    5. Raman and surface enhanced Raman scattering of a black dyed silk (pages 1238–1245)

      T. Aguayo, C. Garrido, R. E. Clavijo, J. S. Gómez-Jeria, C. Araya Monasterio, M. Icaza, F. Espinoza Moraga and M. M. Campos Vallette

      Version of Record online: 12 JUL 2013 | DOI: 10.1002/jrs.4348

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      Raman and surface enhanced Raman scattering spectra of a black dyed silk sample were registered, and the spectral analysis was performed on the basis of spectra obtained from isolated samples of bombyx mori silk fibroin, reactive black 5 dye and the silk's motive peptide GAGAGS. The conformation of the silk, the peptide and the dye on a silver surface is proposed.

    6. Validation of isotope dilution surface-enhanced Raman scattering (IDSERS) as a higher order reference method for clinical measurands employing international comparison schemes (pages 1246–1252)

      Sabine Zakel, Stefan Wundrack, Gavin O'Connor, Bernd Güttler and Rainer Stosch

      Version of Record online: 25 JUL 2013 | DOI: 10.1002/jrs.4349

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      The practical applicability of isotope dilution surface-enhanced Raman scattering (IDSERS) for SI-traceable determination of clinical measurands was demonstrated through successful participation in two international laboratory comparisons. Creatinine concentrations measured in human blood serum agree excellently with data obtained by isotope dilution mass spectrometry. These results led to the approval of IDSERS as a higher order reference measurement procedure, which is now listed in a publicly accessible database maintained by the International Bureau of Weights and Measures (BIPM).

    7. Immune recognition construct plasmonic dimer for SERS-based bioassay (pages 1253–1258)

      Lei Chen, Zhi Yu, Haibo Li, Xu Wang, Chun Zhao, Weiqing Xu, Bing Zhao and Young Mee Jung

      Version of Record online: 15 AUG 2013 | DOI: 10.1002/jrs.4361

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      We have first time demonstrated the construction of a plasmonic gold dimer model for bioassays based on immune recognition with surface-enhanced Raman scattering (SERS). To induce a strong plasmonic coupling effect, a dimer of gold nanoparticles (NPs) with a Raman label located between adjacent NPs is assembled through specific recognition in biological systems.

    8. Efficient excitation of Raman coherence by a gradient force (pages 1259–1262)

      Dongxia Ma and Yuri V. Rostovtsev

      Version of Record online: 21 JUL 2013 | DOI: 10.1002/jrs.4353

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      The vibrational molecular excitation by strong laser fields have been studied. Starting with the molecular Hamiltonian, we derive the vibrational excitation rate due to a gradient of laser field and show that the rate can be two to three orders of magnitude larger than the regular Raman rates. The developed theory contributes to understanding of interaction of powerful laser pulses with molecular systems, and it might be used in developing quantitative coherent Raman spectroscopy.

    9. Pump power dependence in resonance femtosecond stimulated Raman spectroscopy (pages 1263–1272)

      Joohyun Lee, J. Reddy Challa and David W. McCamant

      Version of Record online: 21 JUL 2013 | DOI: 10.1002/jrs.4354

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      The power dependence of resonance femtosecond stimulated Raman spectroscopy (FSRS) was observed and simulated with a photophysical kinetic model. For strongly absorbing molecular systems, the ground-state (GS) FSRS signal can actually decrease with increasing pump power, due to depletion of the GS population. The results are analyzed to provide simple guidelines for acceptable Raman-pump powers in resonance FSRS experiments.

    10. Darling–Dennison resonance of thiourea adsorbed on the silver electrode revealed by surface enhanced Raman spectroscopy (pages 1273–1276)

      Peijie Wang and Zhaojun Liu

      Version of Record online: 29 AUG 2013 | DOI: 10.1002/jrs.4358

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      Darling-Dennison resonance indicates that two degenerate Raman modes interact with each other and their degenerate first-order overtone modes obtain energy and appear in Raman spectra. The surface-enhanced Raman spectroscopy (SERS) of thiourea on Ag electrode excited at 514.5 nm laser excitation was obtained, and the combinational modes, which attributed to Darling-Dennison resonance, were observed in SERS on electrode and on silver island film, respectively, because of strong anharmonicity.

    11. In-situ spectroscopic assessment of the conservation state of building materials from a Palace house affected by infiltration water (pages 1277–1284)

      Olivia Gómez-Laserna, María Ángeles Olazabal, Héctor Morillas, Nagore Prieto-Taboada, Irantzu Martinez-Arkarazo, Gorka Arana and Juan Manuel Madariaga

      Version of Record online: 27 AUG 2013 | DOI: 10.1002/jrs.4359

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      The suitability of a combination of in-situ analysis by means of portable X-Ray fluorescence and Raman spectrometers was evaluated for the diagnosis of the decaying building materials and to obtain further information of the chemical processes involved in their respective affections. In this way, the finding of nitrate salts evidenced the infiltration waters as the main cause of the decaying.

    12. Use of a handheld Raman spectrometer for fast screening of microbial pigments in cultures of halophilic microorganisms and in microbial communities in hypersaline environments in nature (pages 1285–1291)

      J. Jehlička and A. Oren

      Version of Record online: 23 AUG 2013 | DOI: 10.1002/jrs.4362

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      A handheld Raman spectrometer with a 532 nm laser was used to detect pigments in autotrophic (cyanobacteria, purple sulfur bacteria) and heterotrophic halophilic microorganisms (Archaea of the family Halobacteriaceae, Salinibacter). Bacterioruberin was detected as the dominant carotenoid in pellets of cells collected from the saltern ponds in Eilat, Israel. Raman analysis of the colored microbial comunities in a benthic gypsum crust in the salterns showed signals consistent with the presence of spirilloxanthin in the red layer, inhabited by purple sulfur bacteria.

    13. Near-field optical enhancement by lead-sulfide quantum dots and metallic nanoparticles for SERS (pages 1292–1298)

      Tanya Hutter, Sumeet Mahajan and Stephen R. Elliott

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/jrs.4347

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      The electromagnetic-field enhancement that is generated by lead-sulfide quantum dots (QDs) is studied using finite-element method. Various combinations of QDs with metallic nanoparticles and substrates are also investigated.

    14. Classification of iron-based inks by means of micro-Raman spectroscopy and multivariate data analysis (pages 1299–1305)

      Giovanna Piantanida, Eva Menart, Marina Bicchieri and Matija Strlič

      Version of Record online: 8 AUG 2013 | DOI: 10.1002/jrs.4351

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      Principal component analysis, LDA and PLS regression were applied on micro-Raman spectra, collected from a broad set of historical iron-based ink samples, previously characterised for the content of organic acids. Multivariate methods allowed discriminating the Raman signal from the noise typical of organic degraded samples. Using LDA, three classes of inks were discriminated. PLS regression allowed to quantitatively correlate Raman features with the content of protocatechuic acid.

    15. Microstructure and thermal residual stress analysis of SiC fiber through Raman spectroscopy (pages 1306–1311)

      Zhiyuan Xiao, Yanqing Yang, Na Jin, Shuai Liu, Xian Luo and Bin Huang

      Version of Record online: 15 AUG 2013 | DOI: 10.1002/jrs.4356

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      Mechanical properties of CVD-SiC fiber are greatly influenced by fiber microstructure and thermal residual stresses introduced by the preparation of the composites. Microstructure and thermal residual stress along SiC fiber radius varies, which makes characterization difficult. Our Raman results indicate that SiC and Si grain size, defects and thermal residual stress varies along fiber radius, meanwhile a buffer zone connecting different SiC coatings processed in separate reactors in fiber is found.

    16. Coherent optical phonons in alexandrite crystal studied by Optical Kerr Effect spectroscopy (pages 1312–1316)

      K. Polok, J. Konarska, B. Ratajska-Gadomska and W. Gadomski

      Version of Record online: 30 JUL 2013 | DOI: 10.1002/jrs.4360

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      Coherent phonons in alexandrite crystals were observed for the first time by means of the Optical Kerr Effect (OKE) spectroscopy. Obtained temperature dependence of their lifetimes and frequencies was used to calculate anharmonic coupling constants. Widths of the homogeneously broadened Raman lines were calculated from the results.

      We showed that OKE is a very good alternative to transient reflection in phonon investigation.