Wet-fractionation of Phaseolus lunatus seeds: partial characterization of starch and protein

Authors

  • David Betancur-Ancona,

    Corresponding author
    1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av Juárez No. 421, Cd Industrial CP 1288, Apdo, Postal 26, Suc Las Fuentes, Mérida, Yucatán, México
    • Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av Juárez No. 421, Cd Industrial CP 1288, Apdo Postal 26, Suc Las Fuentes, Mérida, Yucatán, Mexico
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  • Santiago Gallegos-Tintoré,

    1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av Juárez No. 421, Cd Industrial CP 1288, Apdo, Postal 26, Suc Las Fuentes, Mérida, Yucatán, México
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  • Luis Chel-Guerrero

    1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av Juárez No. 421, Cd Industrial CP 1288, Apdo, Postal 26, Suc Las Fuentes, Mérida, Yucatán, México
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Abstract

The optimum extraction conditions for integral use of Phaseolus lunatus seed, in alkaline medium, are 1:6 (w/v) flour:water ratio, pH 11 and a 1 h extraction time. Three main fractions were produced under these conditions: starch; protein isolate and fibrous residue by-product. The yield is 288.4 g kg−1 starch, 188.2 g kg−1 protein isolate and the remaining quantity, fibrous residue. The starch has 98.4% purity, a 75 °C gelatinization temperature and high syneresis even at high concentrations. It also has high viscosity, good stability and middle retrogradation during the heating–cooling cycle. The protein isolate contains 711.3 g kg−1 protein as well as 75.5 g lysine kg−1 protein, 10.1 g methionine kg−1 protein and 12.2 g tryptophan kg−1 protein. Its in vitro digestibility is 79% with a 2.5 c-PER. The fibrous residue contains 63 g kg−1 of protein, 328.4 g kg−1 of crude fiber and 567.3 g kg−1 of NFE. Copyright © 2004 Society of Chemical Industry

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