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Bioavailability of a bilberry anthocyanin extract and its impact on plasma antioxidant capacity in rats

Authors

  • Séverine Talavéra,

    Corresponding author
    1. Laboratoire de Pharmacognosie, Faculté de Pharmacie, 28 place Henri Dunant, 63001 Clermont-Ferrand, France
    • Laboratoire de Pharmacognosie, Faculté de Pharmacie, 28 place Henri Dunant, BP38, 63001 Clermont-Ferrand Cedex1, France
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  • Catherine Felgines,

    1. Laboratoire de Pharmacognosie, Faculté de Pharmacie, 28 place Henri Dunant, 63001 Clermont-Ferrand, France
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  • Odile Texier,

    1. Laboratoire de Pharmacognosie, Faculté de Pharmacie, 28 place Henri Dunant, 63001 Clermont-Ferrand, France
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  • Catherine Besson,

    1. Laboratoire des Maladies Métaboliques et des Micronutriments, Institut National de la Recherche Agronomique de Clermont-Ferrand/Theix, 63122 Saint-Genès Champanelle, France
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  • André Mazur,

    1. Laboratoire des Maladies Métaboliques et des Micronutriments, Institut National de la Recherche Agronomique de Clermont-Ferrand/Theix, 63122 Saint-Genès Champanelle, France
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  • Jean-Louis Lamaison,

    1. Laboratoire de Pharmacognosie, Faculté de Pharmacie, 28 place Henri Dunant, 63001 Clermont-Ferrand, France
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  • Christian Rémésy

    1. Laboratoire des Maladies Métaboliques et des Micronutriments, Institut National de la Recherche Agronomique de Clermont-Ferrand/Theix, 63122 Saint-Genès Champanelle, France
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Abstract

Anthocyanins are secondary metabolites from the flavonoid family, frequently found in fruits and vegetables. They have been shown to possess beneficial health effects and various in vitro assays have highlighted their powerful antioxidant capacity. However, little is known about their metabolism after digestion and their antioxidant potency in vivo. The aim of this work was to evaluate anthocyanin bioavailability and to determine the impact of an anthocyanin-rich diet on plasma antioxidant status in rats. Animals were fed for 8 days with a vitamin E-deficient diet supplemented with a bilberry (Vaccinium myrtillus L.) anthocyanin extract providing daily 1.43 mmol anthocyanins kg−1 body weight. An anthocyanin-enriched diet significantly enhanced the plasma antioxidant capacity compared with a control diet (P < 0.0001). Moreover, anthocyanins were recovered in urine in the intact glycosidic forms in addition to unknown metabolites. Urinary excretion of bilberry anthocyanins and their metabolites was 0.71 ± 0.08 µmol per 24 h (ie 0.22% of the ingested dose). Anthocyanins and their corresponding aglycones were detected in caecal contents. After a single oral administration of the bilberry extract, native anthocyanins quickly (30 min) appeared in plasma. Hence, in spite of a low bioavailability, bilberry anthocyanin extract consumption has a positive effect on plasma antioxidant capacity. Copyright © 2005 Society of Chemical Industry

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