Results are presented for the levels of arsenic, cadmium, chromium, cobalt, copper, lead, manganese, mercury, molybdenum, nickel, selenium and zinc, as determined by atomic absorption spectrophotometry, in the livers and kidneys of 190 cattle from eight different locations in New South Wales. The mean and range for each of the trace metals, in parts 10−6, in livers and kidneys, respectively were as follows: arsenic 0.013 (n.d.−0.09) and 0.018 (n.d.−0.10); cadmium 0.08 (n.d.−0.80) and 0.43(0.02–10.7); chromium 0.05 (n.d.-1.0) and 0.03 (n.d.−0.22); cobalt 0.07 (n.d.−0.45) and 0.03 (n.d.−0.25); copper 18.0(0.81–82.8) and 3.9 (1.84–9.2); lead 0.14(n.d.−0.85) and 0.22(n.d.-2.29); manganese 2.72(1.11–4.09) and 1.07(0.58–1.67); mercury 0.005 (n.d.−0.050) and 0.006 (n.d.−0.177); molybdenum 0.88 (n.d.-2.41) and 0.31 (n.d.-1.85); nickel 0.33 (n.d.-4.82) and 0.46 (n.d.-5.80); selenium 0.17(0.01–0.63) and 1.10 (0.47–1.77); zinc 37.5 (13.4–99.2) and 18.6 (12.9–31.6). Significant differences were observed in the trace metal levels in livers and kidneys from the different regions of the State and between the trace metal levels in livers and kidneys from the same region. The accuracy of the methods was confirmed by analysis of NBS No. 1577 Bovine Liver.