This research was presented in part at the 57th Annual Meeting of the Canadian Society of Microbiologists, Quebec, Canada, 17–20 June 2007
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Research Article
The effect of glyphosate on digestion and horizontal gene transfer during in vitro ruminal fermentation of genetically modified canola†
Article first published online: 21 AUG 2007
DOI: 10.1002/jsfa.3038
Copyright © 2007 Society of Chemical Industry
Issue
1097-0010/asset/cover.gif?v=1&s=b47620dd25eabc179c6838560a664c2310e44227 "Journal of the Science of Food and Agriculture")
Journal of the Science of Food and Agriculture
Volume 87, Issue 15, pages 2837–2843, December 2007
Additional Information
How to Cite
Reuter, T., Alexander, T. W., Martínez, T. F. and McAllister, T. A. (2007), The effect of glyphosate on digestion and horizontal gene transfer during in vitro ruminal fermentation of genetically modified canola. J. Sci. Food Agric., 87: 2837–2843. doi: 10.1002/jsfa.3038
- †
Publication History
- Issue published online: 9 NOV 2007
- Article first published online: 21 AUG 2007
- Manuscript Accepted: 2 JUN 2007
- Manuscript Revised: 18 MAY 2007
- Manuscript Received: 21 MAR 2007
Funded by
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Food Inspection Agency
- Abstract
- Article
- References
- Cited By
Keywords:
- genetically modified canola;
- glyphosate;
- horizontal gene transfer;
- rumen bacteria;
- fermentation
Abstract
BACKGROUND: The impact of glyphosate on ruminal fermentation, selective pressure on ruminal bacteria and horizontal transfer of the gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (epsps) to ruminal bacteria was studied using batch culture with glyphosate-tolerant (Roundup Ready®) canola meal as substrate.
RESULTS: A glyphosate concentration × time interaction (P < 0.05) occurred when glyphosate (0–100 mmol L−1) was included in in vitro ruminal incubations with a diet containing 150 g kg−1 Roundup Ready® canola meal (Experiment 1). Glyphosate at 50 and 100 mmol L−1 inhibited fermentation. In Experiment 2, epsps fragments were detected in plant debris for up to 16 h of incubation using primer sets that amplified three fragments (62, 108 and 300 bp) of DNA spanning the transgenic construct. Persistence was affected by fragment size but not by glyphosate concentration (0, 10 or 60 mmol L−1). Extensive polymerase chain reaction assays provided no evidence of acquisition of epsps by feed- or fluid-associated bacteria during fermentation. A glyphosate concentration × time interaction (P < 0.05) was observed for all fermentation parameters measured, and glyphosate caused a general inhibition of fermentation.
CONCLUSION: The presence of glyphosate did not increase selective pressure for gene transfer of DNA encoding glyphosate resistance from Roundup Ready® canola meal to ruminal bacteria. Copyright © 2007 Society of Chemical Industry