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Effects of extracts of spices on rumen methanogenesis, enzyme activities and fermentation of feeds in vitro

Authors

  • Amlan Kumar Patra,

    Corresponding author
    1. Centre of Advanced Studies in Animal Nutrition, Rumen Microbiology Lab, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP 243122, India
    • Department of Animal Nutrition, West Bengal University of Animal and Fishery Sciences, 37, K. B. Sarani, Belgachia, Kolkata 700037, India.
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  • Devki Nandan Kamra,

    1. Centre of Advanced Studies in Animal Nutrition, Rumen Microbiology Lab, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP 243122, India
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  • Neeta Agarwal

    1. Centre of Advanced Studies in Animal Nutrition, Rumen Microbiology Lab, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP 243122, India
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  • Part (total gas and methane production) of the data has been published in the International Congress Series (2006, vol. 1293, pp. 176–179): Greenhouse Gases and Animal Agriculture: An Update. Proceedings of the Second International Conference on Greenhouse Gases and Animal Agriculture, held in Zurich, Switzerland, between 20 and 24 September 2005.

Abstract

BACKGROUND: An experiment was conducted to study the effects of boiling water, methanol and ethanol extracts (0, 0.25 and 0.50 mL) of seeds of Foeniculum vulgare (fennel), flower buds of Syzygium aromaticum (clove), bulbs of Allium sativum (garlic), bulbs of Allium cepa (onion) and roots of Zingiber officinalis (ginger) on rumen methanogenesis, fibrolytic enzyme activities and fermentation characteristics in vitro.

RESULTS: Ethanol and methanol extracts of fennel, clove and garlic at 0.50 mL and clove at 0.25 mL inhibited (P < 0.05) methane production. Carboxymethylcellulase activity was reduced (P < 0.05) by ethanol and methanol extracts (0.50 mL) of fennel and clove (0.25 and 0.50 mL). The extracts of clove reduced (0.25 and 0.50 mL) xylanase and acetylesterase activities, and the fennel extract (0.50 mL) reduced (P < 0.05) xylanase activity. However, the extracts of garlic (0.50 mL) increased (P < 0.05) acetylesterase activity. Concentrations of volatile fatty acids were reduced (P < 0.05) by the extracts of garlic and onion. The extracts of garlic caused a decrease (P < 0.05) in acetate:propionate ratio (A:P) at 0.50 mL, whereas A:P was increased (P < 0.05) by the inclusion of 0.50 mL extracts of clove. Methanol and ethanol extracts of clove decreased (P < 0.05) in vitro organic matter degradability. Extracts (0.50 mL) of clove decreased (P < 0.05) the numbers of total protozoa, small entodiniomorphs and holotrichs, whereas extracts of onion, ginger and garlic enhanced (P < 0.05) protozoal numbers (both entodiniomorphs and holotrichs).

CONCLUSION: Ethanol and methanol extracts of fennel and garlic have potential to inhibit rumen methanogenesis without adversely affecting rumen fermentation. Copyright © 2009 Society of Chemical Industry

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