• pine needle;
  • volatile compound;
  • antibacterial;
  • antioxidant;
  • Pinus densiflora


BACKGROUND: To investigate the volatile compounds and the antibacterial and antioxidant effects of the essential oils of Pinus densiflora needles (EPDN) and Pinus thunbergii needles (EPTN), the volatile compounds of steam-distilled essential oils were analysed by gas chromatography–mass spectrometry. Antibacterial activities were analysed by performing disc-agar diffusion assay and determining the minimum inhibitory concentrations (MICs) of the essential oils. Antioxidant activities were analysed via radical- and nitrite-scavenging activity assays.

RESULTS: The yields of EPDN and EPTN were 0.304% (v/w) and 0.296% (v/w), respectively. In the antibacterial activity assay, the MICs of EPDN and EPTN for Klebsiella pneumoniae, Shigella flexneri and Proteus vulgaris were < 0.4 mg mL−1. In the antioxidant activity assay, the 50% inhibitory concentrations (IC50) of EPDN and EPTN were 120 and 30 µg mL−1, respectively. At 1680 µg mL−1, both EPDN and EPTN exhibited > 50% nitrite-scavenging activity.

CONCLUSION: EPDN can be used as a natural antimicrobial substance. Copyright © 2011 Society of Chemical Industry