Comparative analysis of the mycobiota and mycotoxins contaminating corn trench silos and silo bags

Authors

  • María L González Pereyra,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
    2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
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  • Stella M Chiacchiera,

    1. Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
    2. Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina
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  • Carlos AR Rosa,

    1. Departamento de Microbiologia e Imunología Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil
    2. Conselho Nacional de Pesquisas Científicas (CNPq), Brasilia, Brazil
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  • Ricardo Sager,

    1. EEA San Luis, Instituto Nacional de Tecnología Agropecuaria (INTA), 5700 San Luis, Argentina
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  • Ana M Dalcero,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
    2. Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina
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  • Lilia Cavaglieri

    Corresponding author
    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5800 Río Cuarto, Córdoba, Argentina
    2. Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina
    • Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km, 601, 5800 Río Cuarto, Córdoba, Argentina.
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Abstract

BACKGROUND: Silage is one of the most important feed sources for bovines. Mycotoxin contamination of feedstuffs is a worldwide concern. The aim of this study was to compare mycobiota and levels of aflatoxin B1 (AFB1), fumonisin B1 (FB1), deoxynivalenol (DON), zearalenone (ZEA) and patulin (PAT) in corn trench silos and silo bags.

RESULTS: Dry matter was higher in trench silos. Counts varied from not detected to 108 CFU g−1 in both trench silos and silo bags. Isolation frequencies of Aspergillus spp. and Fusarium spp. were higher in trench silos, whereas Penicillium spp. was higher in silo bags. Silo bags showed less diversity than trench silos. Strains isolated produced AFB1, FB1 and PAT. In trench silos, AFB1 was the only mycotoxin detected (1–160 µg kg−1). In silo bags AFB1 levels varied from 5.8 to 47.4 µg kg−1. DON was detected in two silo bag samples.

CONCLUSION: When handling is adequate the reduction of mould and mycotoxin contamination in silo bags is considerable. This study will enable estimation of the mycotoxicological risk of different ensiling practices and determination of the most adequate method to minimize economic losses and reduce hazard to animal and human health. Copyright © 2011 Society of Chemical Industry

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