Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting
Article first published online: 14 SEP 2011
Copyright © 2011 Society of Chemical Industry
Journal of the Science of Food and Agriculture
Volume 92, Issue 3, pages 660–666, February 2012
How to Cite
Lee, C. M., Sieo, C. C., Cheah, Y.-K., Abdullah, N. and Ho, Y. W. (2012), Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting. J. Sci. Food Agric., 92: 660–666. doi: 10.1002/jsfa.4627
- Issue published online: 4 JAN 2012
- Article first published online: 14 SEP 2011
- Manuscript Accepted: 14 JUL 2011
- Manuscript Revised: 30 APR 2011
- Manuscript Received: 4 JAN 2011
- Lactobacillus spp.;
BACKGROUND: Four repetitive element sequence-based polymerase chain reaction (rep-PCR) methods, namely repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), polytrinucleotide (GTG)5-PCR and BOX-PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep-PCR methods and their combination (composite rep-PCR) in the molecular typing of Lactobacillus strains based on a discriminatory index (D).
RESULTS: Species-specific and strain-specific profiles were observed from rep-PCR. From the numerical analysis of composite rep-PCR, BOX-PCR, (GTG)5-PCR, REP-PCR and ERIC-PCR, D values of 0.9118, 0.9044, 0.8897, 0.8750 and 0.8529 respectively were obtained. Composite rep-PCR analysis was the most discriminative method, with eight Lactobacillus strains, namely L. brevis ATCC 14869T, L. reuteri C 10, L. reuteri ATCC 23272T, L. gallinarum ATCC 33199T, L. salivarius ATCC 11741T, L. salivarius I 24, L. panis JCM 11053T and L. panis C 17, being differentiated at the strain level.
CONCLUSION: Composite rep-PCR analysis is potentially a useful fingerprinting method to discriminate probiotic Lactobacillus strains isolated from the gastrointestinal tract of chickens. Copyright © 2011 Society of Chemical Industry