Assessment of antibacterial properties and the active ingredient of plant extracts and its effect on the performance of crucian carp (Carassius auratus gibelio var. E'erqisi, Bloch)

Authors

  • Chunxia Lu,

    Corresponding author
    • Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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  • Xiaoling Luo,

    1. Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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  • Ruifeng Luo,

    1. Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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  • Xia Chen,

    1. Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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  • Lijie Xing,

    1. Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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  • Zonggui Tang,

    1. Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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  • Hongmin Li

    1. Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China
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Correspondence to: Chunxia Lu, Supervision and Testing Center Food Quality, Ministry of Agriculture, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi, 832000, P.R. China. E-mail: shzlcx2002@163.com

Abstract

BACKGROUND: In this study, the antibacterial properties and active ingredient of plant extracts and its effect on the performance of crucian carp (Carassius auratus gibelio var. E'erqisi, Bloch) were assessed.

RESULTS: The transmission electron microscopy and flow cytometric analysis showed that the antibacterial activity of plant extracts is due to the disruption of the cell membrane and the leakage of cytoplasmic contents. The UPLC–MS/MS analysis showed that the contents of gallic acid, (−)–epigallocatechin, (+)–catechin, (−)–epigallocatechin gallate, (−)–epicatechin gallate, aloe–emodin, rhein, emodin, chrysophanol, and physcion, were 5.27%, 3.30%, 1.08%, 19.32%, 5.46%, 0.23%, 0.56%, 1.28%, 0.75% and 0.39% in plant extracts, respectively. Results of feeding experiment showed that feeding crucian carp with 1.0% and 2.0% plant extracts significantly enhanced specific growth rate, serum total protein, lysozyme, catalase and superoxide dismutase activities, and decreased the feed conversion rate, malondialdehyde contents and the mortality rate (P < 0.05).

CONCLUSION: It can be concluded that plant extracts added to fish feed can act as natural antimicrobial and immunostimulants to prevent pathogenic infection, enhance immune response, and promote growth of the fish. © 2012 Society of Chemical Industry

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