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Angiotensin-converting enzyme-inhibitory activity in protein hydrolysates from normal and anthracnose disease-damaged Phaseolus vulgaris seeds

Authors

  • Alan Javier Hernández-Álvarez,

    1. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, Mexico
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  • Janet Carrasco-Castilla,

    1. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, Mexico
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  • Gloria Dávila-Ortiz,

    1. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, Mexico
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  • Manuel Alaiz,

    1. Department of Physiology and Technology of Plant Products, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, E-41012 Seville, Spain
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  • Julio Girón-Calle,

    1. Department of Physiology and Technology of Plant Products, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, E-41012 Seville, Spain
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  • Javier Vioque-Peña,

    1. Department of Physiology and Technology of Plant Products, Instituto de la Grasa, Consejo Superior de Investigaciones Científicas, E-41012 Seville, Spain
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  • Carmen Jacinto-Hernández,

    1. Campo Experimental Valle de México (CEVAMEX), Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Carretera Los Reyes-Texcoco, km 13.5, Chapingo, 56250 Texcoco, Estado de México, Mexico
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  • Cristian Jiménez-Martínez

    Corresponding author
    • Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, Mexico
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Correspondence to: Cristian Jiménez-Martínez, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio, Esq. Plan de Ayala S/N, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, Mexico. E-mail: crisjm_99@yahoo.com

Abstract

BACKGROUND: Bean seeds are an inexpensive source of protein. Anthracnose disease caused by the fungus Colletotrichum lindemuthianum results in serious losses in common bean (Phaseolus vulgaris L.) crops worldwide, affecting any above-ground plant part, and protein dysfunction, inducing the synthesis of proteins that allow plants to improve their stress tolerance. The aim of this study was to evaluate the use of beans damaged by anthracnose disease as a source of peptides with angiotensin-converting enzyme (ACE-I)-inhibitory activity.

RESULTS: Protein concentrates from beans spoiled by anthracnose disease and from regular beans as controls were prepared by alkaline extraction and precipitation at isolelectric pH and hydrolysed using Alcalase 2.4 L. The hydrolysates from spoiled beans had ACE-I-inhibitory activity (IC50 0.0191 mg protein mL−1) and were very similar to those from control beans in terms of ACE-I inhibition, peptide electrophoretic profile and kinetics of hydrolysis. Thus preparation of hydrolysates using beans affected by anthracnose disease would allow for revalorisation of this otherwise wasted product.

CONCLUSION: The present results suggest the use of spoiled bean seeds, e.g. anthracnose-damaged beans, as an alternative for the isolation of ACE-I-inhibitory peptides to be further introduced as active ingredients in functional foods. © 2012 Society of Chemical Industry

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