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Keywords:

  • fumonisins;
  • high-performance liquid chromatography;
  • Chinese rice wine

Abstract

BACKGROUND: Fumonisins are a group of naturally occurring mycotoxins produced by various Fusarium species that commonly infect maize and other cereals, including sorghum and rice. In this study a sensitive and selective method was developed for the determination of fumonisins B1 and B2 (FB1 and FB2) in Chinese rice wine. The method is based on high-performance liquid chromatography and fluorescence detection following precolumn derivatisation with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC).

RESULTS: FB1 and FB2 in Chinese rice wine were extracted and purified using strong anion exchange cartridges and derivatised with AQC at room temperature. The AQC derivatives were stable for 5 days. Optimal fluorescence was obtained at an excitation wavelength of 246 nm and an emission wavelength of 390 nm. Chromatography was performed using a C18 column and gradient elution at 1 mL min−1 with methanol and 0.05 mol L−1 phosphate buffer at pH 4. The limit of detection was 6 µg L−1 for both FB1 and FB2. The method was successfully applied to the determination of FB1 and FB2 in Chinese rice wine, with recoveries of 87.5–94.5% being obtained.

CONCLUSION: The established method was stable and sensitive for the determination of FB1 and FB2 in Chinese rice wine. © 2012 Society of Chemical Industry