Yan Z Liu and Guo X Zhao contributed equally to this study.
Synthesis of novel haptens against ciprofloxacin and production of generic monoclonal antibodies for immunoscreening of fluoroquinolones in meat
Article first published online: 9 OCT 2012
© 2012 Society of Chemical Industry
Journal of the Science of Food and Agriculture
Volume 93, Issue 6, pages 1370–1377, April 2013
How to Cite
Liu, Y. Z., Zhao, G. X., Liu, J., Zhang, H. C., Wang, P. and Wang, J. P. (2013), Synthesis of novel haptens against ciprofloxacin and production of generic monoclonal antibodies for immunoscreening of fluoroquinolones in meat. J. Sci. Food Agric., 93: 1370–1377. doi: 10.1002/jsfa.5901
- Issue published online: 21 MAR 2013
- Article first published online: 9 OCT 2012
- Accepted manuscript online: 6 SEP 2012 04:46AM EST
- Manuscript Accepted: 6 SEP 2012
- Manuscript Revised: 29 JUL 2012
- Manuscript Received: 11 JUN 2012
- monoclonal antibody;
- heterologous ELISA;
The residues of fluoroquinolone drugs in foods of animal origin are dangerous to the consumers. The objective of this study was to produce a generic monoclonal antibody for determination of fluoroquinolone residues in meat.
Two novel haptens of ciprofloxacin containing a free amidogen group on the piperazinyl ring were synthesised that were used to produce the monoclonal antibodies. The antibodies obtained simultaneously recognised 12 fluoroquinolones (ciprofloxacin, enrofloxacin, norfloxacin, sarafloxacin, diflocaxin, danofloxacin, ofloxacin, marbofloxacin, pefloxacin, lomefloxacin, amifloxacin and enofloxacin). After evaluation of different coating antigen–antibody combinations, a heterologous competitive indirect ELISA was used to determine the 12 drugs. The cross-reactivities were in the range of 23–120% and the limits of detection were in the range of 1.0–4.5 ng mL−1. Eight fluoroquinolone drugs licensed as veterinary drugs in China were fortified into blank chicken for analysis. The recoveries were in the range of 61.5–82.5% with coefficients of variation in the range of 7.5–15.2%.
This method could be used as a rapid screening tool for routine monitoring the residues of these fluoroquinolone drugs in animal-derived foods.