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Synthesis of novel haptens against ciprofloxacin and production of generic monoclonal antibodies for immunoscreening of fluoroquinolones in meat

Authors

  • Yan Z Liu,

    1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China 071000
    2. Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China 071000
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    • Yan Z Liu and Guo X Zhao contributed equally to this study.

  • Guo X Zhao,

    1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China 071000
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    • Yan Z Liu and Guo X Zhao contributed equally to this study.

  • Jing Liu,

    1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China 071000
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  • Hui C Zhang,

    1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China 071000
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  • Ping Wang,

    1. Hebei Institute of Veterinary Drug Control, Shijiazhuang Hebei, China 050051
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  • Jian P Wang

    Corresponding author
    1. Hebei Engineering and Technology Research Center of Veterinary Biological Products, Agricultural University of Hebei, Baoding Hebei, China 071000
    • College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China 071000
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Correspondence to: Jian P Wang,

College of Veterinary Medicine, Agricultural University of Hebei, Baoding Hebei, China 071000. E-mail: wjpcau@yahoo.com.cn

Abstract

Background

The residues of fluoroquinolone drugs in foods of animal origin are dangerous to the consumers. The objective of this study was to produce a generic monoclonal antibody for determination of fluoroquinolone residues in meat.

Results

Two novel haptens of ciprofloxacin containing a free amidogen group on the piperazinyl ring were synthesised that were used to produce the monoclonal antibodies. The antibodies obtained simultaneously recognised 12 fluoroquinolones (ciprofloxacin, enrofloxacin, norfloxacin, sarafloxacin, diflocaxin, danofloxacin, ofloxacin, marbofloxacin, pefloxacin, lomefloxacin, amifloxacin and enofloxacin). After evaluation of different coating antigen–antibody combinations, a heterologous competitive indirect ELISA was used to determine the 12 drugs. The cross-reactivities were in the range of 23–120% and the limits of detection were in the range of 1.0–4.5 ng mL−1. Eight fluoroquinolone drugs licensed as veterinary drugs in China were fortified into blank chicken for analysis. The recoveries were in the range of 61.5–82.5% with coefficients of variation in the range of 7.5–15.2%.

Conclusion

This method could be used as a rapid screening tool for routine monitoring the residues of these fluoroquinolone drugs in animal-derived foods.

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