Wei-Jun Kong and Hong-Hong Shen contributed equally to this work.
Analysis of zearalenone and α-zearalenol in 100 foods and medicinal plants determined by HPLC-FLD and positive confirmation by LC-MS-MS
Article first published online: 24 OCT 2012
© 2012 Society of Chemical Industry
Journal of the Science of Food and Agriculture
Volume 93, Issue 7, pages 1584–1590, May 2013
How to Cite
Kong, W.-J., Shen, H.-H., Zhang, X.-F., Yang, X.-L., Qiu, F., Ou-yang, Z. and Yang, M.-H. (2013), Analysis of zearalenone and α-zearalenol in 100 foods and medicinal plants determined by HPLC-FLD and positive confirmation by LC-MS-MS. J. Sci. Food Agric., 93: 1584–1590. doi: 10.1002/jsfa.5926
- Issue published online: 11 APR 2013
- Article first published online: 24 OCT 2012
- Accepted manuscript online: 19 SEP 2012 03:34AM EST
- Manuscript Accepted: 19 SEP 2012
- Manuscript Revised: 8 SEP 2012
- Manuscript Received: 20 JUN 2012
- foods and medicinal plants;
Mycotoxins, which may contaminate many foods and medicinal plants, are poisonous to humans. A high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method was successfully developed for analysing the contamination levels of zearalenone (ZON) and its metabolite α-zearalenol (α-ZOL) in 100 widely consumed foods and medicinal plants in China. Samples were extracted with methanol–water (80:20, v/v), and cleaned up by using an immunoaffinity column.
The limits of detection of this developed method for ZON and α-ZOL were 4 µg kg−1 and 2.5 µg kg−1, respectively. Recoveries for the samples spiked with three levels (30, 60 and 300 µg kg−1 for ZON and α-ZOL) ranged from 85.8% to 96.1% with relative standard deviation (RSD) of 2.6–7.1% for ZON, and from 89.9% to 98.7% with RSD of 1.9–9.2% for α-ZOL. Twelve (12%) of these tested samples were contaminated with ZON at levels ranging from 5.3 to 295.8 µg kg−1. The most contaminated samples were Semen coicis, four of them in a concentration level exceeding 60 µg kg−1 ‘maximum level’ (range 68.9–119.6 µg kg−1). Positive samples were further confirmed by liquid chromatography–tandem mass spectrometry.
The results suggest that it is necessary to control ZON contamination in medicinal plants, especially Semen coicis. This is a successful study on the analysis of ZON and α-ZOL in medicinal plants in China by HPLC-FLD. Immunoaffinity clean-up and HPLC-FLD proved to have broad applicability in the field of simultaneously detecting ZON and α-ZOL in foods and medicinal plants and other complicated matrices.© 2012 Society of Chemical Industry