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Research Article
FT-IR and NMR study of seed coat dissected from different colored progenies of Brassica napus–Sinapis alba hybrids
Article first published online: 12 DEC 2012
DOI: 10.1002/jsfa.5986
© 2012 Society of Chemical Industry
Additional Information
How to Cite
Jiang, J., Shao, Y., Li, A., Zhang, Y., Wei, C. and Wang, Y. (2013), FT-IR and NMR study of seed coat dissected from different colored progenies of Brassica napus–Sinapis alba hybrids. J. Sci. Food Agric., 93: 1898–1902. doi: 10.1002/jsfa.5986
Publication History
- Issue published online: 8 MAY 2013
- Article first published online: 12 DEC 2012
- Accepted manuscript online: 21 NOV 2012 07:32AM EST
- Manuscript Accepted: 21 NOV 2012
- Manuscript Revised: 22 OCT 2012
- Manuscript Received: 20 JUL 2012
- Abstract
- Article
- References
- Cited By
Keywords:
- Fourier transform infrared (FT-IR) spectroscopy;
- nuclear magnetic resonance (NMR);
- yellow seed;
- black seed;
- seed coat;
- chemical composition
Abstract
BACKGROUND
Yellow-seeded Brassica napus is preferred by breeders for economic reasons, and seed coat is the most obvious comparable character between yellow and black rapeseed.
RESULTS
Owing to an incomplete understanding of chemical components in yellow rapeseed coat, here we optimized a technique based on attenuated total reflectance–Fourier transform infrared (FT-IR) microspectroscopy to screen differences in chemical compositions of Brassica napus, Sinapis alba and four progeny lines with different yellow seed color derived from somatic hybrids between B. napus and S. alba. Nuclear magnetic resonance (NMR) analysis of seed coat from B. napus and S. alba represented two specific peaks representing components of lignin in B. napus, which were absent in the progenies. Also, the intensity of peaks related to fiber were stronger in B. napus than S. alba and yellow seed lines, indicating the differences of fiber and lignin contents in yellow-seeded lines and parent lines. Differences in the lipid, protein and carbohydrate features were identified between B. napus and S. alba, some of which were specifically inherited in progenies of the somatic hybrids.
CONCLUSION
FT-IR spectrometry and NMR provide a rapid, direct bioanalytical technique for exploring the changes in molecular chemistry within the canola seed coat and for selecting rapeseed materials with high quality, which can be further used for breeding or nutrition of human and animals. © 2012 Society of Chemical Industry