Role of Adhatoda vasica (L.) Nees leaf extract in the prevention of aflatoxin-induced toxicity in Wistar rats

Authors

  • Rajendran Brinda,

    1. Department of Food and Agricultural Processing Engineering, Agricultural Engineering College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    Search for more papers by this author
  • Selvaraj Vijayanandraj,

    1. Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    Search for more papers by this author
  • Doraiswamy Uma,

    1. Department of Bio-Energy, Agricultural Engineering College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    Search for more papers by this author
  • Dorairaj Malathi,

    1. Post Harvest Technology Centre, Agricultural Engineering College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    Search for more papers by this author
  • Vaikuntavasan Paranidharan,

    1. Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    Search for more papers by this author
  • Rethinasamy Velazhahan

    Corresponding author
    1. Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    • Department of Food and Agricultural Processing Engineering, Agricultural Engineering College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
    Search for more papers by this author

Correspondence to: Rethinasamy Velazhahan, Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu, India. E-mail: velazhahan@hotmail.com

Abstract

Background

Aflatoxin contamination of various foodstuffs and agricultural commodities is a major problem worldwide. Several strategies have been reported for the detoxification of aflatoxins in contaminated foods and feeds, but all these methods have their own shortcomings. Traditional medicinal plants are potential sources of aflatoxin-detoxifying compounds. In this study a spray-dried formulation of Adhatoda vasica (L.) Nees leaf extract was prepared and its chemopreventive effect on aflatoxin B1 (AFB1)-induced biochemical changes in the liver and serum of Wistar rats was investigated.

Results

Administration of AFB1 (1.5 mg kg−1 body weight (BW) intraperitoneally) to rats significantly reduced the activities of superoxide dismutase and catalase in liver tissues and increased the activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase and the levels of very-low-density lipoprotein, low-density lipoprotein and cholesterol in blood serum. However, pre-feeding of rats with A. vasica formulation (500 mg kg−1 BW for 7 days) protected the animals from AFB1-induced biochemical changes during subsequent exposure to AFB1.

Conclusion

Pre-feeding of rats with A. vasica formulation counteracted the hepatic dysfunction induced by subsequent treatment with AFB1. This formulated A. vasica extract offers a biologically safe alternative to detoxify aflatoxin and has huge potential to be used in the poultry industry to reduce aflatoxicosis. © 2013 Society of Chemical Industry

Ancillary