Inhibitory effect of unicellular green algae (Chlorella vulgaris) water extract on allergic immune response

Authors

  • Min-Jung Bae,

    1. Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seognam-si, Kyeonggi-do, Republic of Korea
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    • These authors contributed equally to this work.
  • Hee Soon Shin,

    1. Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seognam-si, Kyeonggi-do, Republic of Korea
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    • These authors contributed equally to this work.
  • Ok Hee Chai,

    1. Department of Anatomy, Medical School and Institute for Medical Sciences, Chonbuk National University, Deokjin-gu, Jeonju-si, Jeollabuk-do, Republic of Korea
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  • Jae-Gab Han,

    1. DAESANG Corp. Health Food R&D Division, 125-8, Pyokyo-Ri, Majang-Myun, Icheon-si, Kyeonggi-do, Republic of Korea
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  • Dong-Hwa Shon

    Corresponding author
    • Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201-62, Anyangpangyo-ro, Bundang-gu, Seognam-si, Kyeonggi-do, Republic of Korea
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Correspondence to: Dong-Hwa Shon, Division of Metabolism and Functionality Research, Korea Food Research Institute, 1201–62, Anyangpangyo-ro, Bundang-gu, Seognam-si, Kyeonggi-do 463-746, Republic of Korea. E-mail: dhs95@kfri.re.kr

Abstract

Background

Chlorella is used as a functional food in East Asia and has been shown to enhance immune system function. However, there has been no direct evidence of the suppressive effect of a hot water extract of Chlorella vulgaris (CVE) on histamine-mediated allergic responses.

Results

The antihistamine activity of CVE was analysed using rat peritoneal mast cells (RPMCs) stimulated by compound 48/80. For in vivo verification, ovalbumin (OVA)-immunised BALB/c mice were treated with CVE orally. Serum immunoglobulin E (IgE) levels and splenocyte cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). CVE prevented histamine release through degranulation of mast cells by blocking the uptake of extracellular Ca2+ into RPMCs. Moreover, CVE administration inhibited serum IgE overproduction by OVA via induction of T helper 1 (Th1) skewing that was dependent on interferon-γ (IFN-γ) and interleukin 12 (IL-12) secretion.

Conclusion

The results of this study clearly demonstrate that CVE acts as an antiallergic dietary agent by suppressing histamine release via its enhancive effect on Th1-related responses. © 2013 Society of Chemical Industry

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