These authors contributed equally to this work.
Inhibitory effect of unicellular green algae (Chlorella vulgaris) water extract on allergic immune response
Article first published online: 5 APR 2013
© 2013 Society of Chemical Industry
Journal of the Science of Food and Agriculture
Volume 93, Issue 12, pages 3133–3136, September 2013
How to Cite
Bae, M.-J., Shin, H. S., Chai, O. H., Han, J.-G. and Shon, D.-H. (2013), Inhibitory effect of unicellular green algae (Chlorella vulgaris) water extract on allergic immune response. J. Sci. Food Agric., 93: 3133–3136. doi: 10.1002/jsfa.6114
- Issue published online: 30 JUL 2013
- Article first published online: 5 APR 2013
- Accepted manuscript online: 20 FEB 2013 03:17PM EST
- Manuscript Accepted: 20 FEB 2013
- Manuscript Revised: 6 FEB 2013
- Manuscript Received: 10 JAN 2013
- Chlorella vulgaris water extract (CVE);
- mast cells;
Chlorella is used as a functional food in East Asia and has been shown to enhance immune system function. However, there has been no direct evidence of the suppressive effect of a hot water extract of Chlorella vulgaris (CVE) on histamine-mediated allergic responses.
The antihistamine activity of CVE was analysed using rat peritoneal mast cells (RPMCs) stimulated by compound 48/80. For in vivo verification, ovalbumin (OVA)-immunised BALB/c mice were treated with CVE orally. Serum immunoglobulin E (IgE) levels and splenocyte cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA). CVE prevented histamine release through degranulation of mast cells by blocking the uptake of extracellular Ca2+ into RPMCs. Moreover, CVE administration inhibited serum IgE overproduction by OVA via induction of T helper 1 (Th1) skewing that was dependent on interferon-γ (IFN-γ) and interleukin 12 (IL-12) secretion.
The results of this study clearly demonstrate that CVE acts as an antiallergic dietary agent by suppressing histamine release via its enhancive effect on Th1-related responses. © 2013 Society of Chemical Industry