Shengjie Li and Tingtao Chen contributed equally to this work.
The beneficial effect of exopolysaccharides from Bifidobacterium bifidum WBIN03 on microbial diversity in mouse intestine
Article first published online: 11 JUL 2013
© 2013 Society of Chemical Industry
Journal of the Science of Food and Agriculture
Volume 94, Issue 2, pages 256–264, 30 January 2014
How to Cite
Li, S., Chen, T., Xu, F., Dong, S., Xu, H., Xiong, Y. and Wei, H. (2014), The beneficial effect of exopolysaccharides from Bifidobacterium bifidum WBIN03 on microbial diversity in mouse intestine. J. Sci. Food Agric., 94: 256–264. doi: 10.1002/jsfa.6244
- Issue published online: 4 DEC 2013
- Article first published online: 11 JUL 2013
- Accepted manuscript online: 28 MAY 2013 12:37PM EST
- Manuscript Accepted: 28 MAY 2013
- Manuscript Revised: 10 MAY 2013
- Manuscript Received: 22 MAR 2013
- exopolysaccharide (EPS);
- Bifidobacterium bifidum WBIN03;
- intestinal microbiota;
- viable cell count
The structure of exopolysaccharides (EPS) produced by Bifidobacterium and their beneficial effects on human health have been fully studied, but only a few studies have investigated their influence on microbial diversity in the human/animal intestine.
The strain named Bifidobacterium bifidum WBIN03 with high growth rate and exopolysaccharide (EPS) yield was selected to study the effect of its EPS on modifying the intestinal microbiota of mice. The results indicated that EPS significantly increased the growth of lactobacilli and total anaerobic bacteria, and exerted their inhibition effect on the growth of enterobacteria, enterococci and Bacteroides fragilis. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that the EPS significantly increased the diversity of total bacteria and lactobacilli, but significantly decreased the diversity of enterobacteria. When receiving a low dose of EPS, Bacteroidales sp./Lactobacillus sp. occupied the dominant position, and L. johnsonii, L. animalis and L. reuteri were identified as the dominant strains when receiving a high concentration of EPS.
The combination of viable cell count, DGGE and sequencing was used as an effective method to assess the microbial diversity in mouse intestine, and the benefit effect of EPS from B. bifidum WBIN03 on probiotics and antagonistic effect against pathogens would guaranteed the health of their hosts. © 2013 Society of Chemical Industry