In vitro and in vivo control of Alternaria alternata in cherry tomato by essential oil from Laurus nobilis of Chinese origin

Authors

  • Shixiang Xu,

    1. Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou, China
    2. Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
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  • Fujie Yan,

    1. Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou, China
    2. Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
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  • Zhendan Ni,

    1. Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou, China
    2. Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
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  • Qianru Chen,

    1. Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou, China
    2. Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
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  • Hui Zhang,

    1. Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou, China
    2. Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
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  • Xiaodong Zheng

    Corresponding author
    1. Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou, China
    2. Fuli Institute of Food Science, Zhejiang University, Hangzhou, China
    • Correspondence to: Xiaodong Zheng, Department of Food Science and Nutrition, Zijingang Campus, Zhejiang University, Hangzhou 310058, China E-mail: foodbiotech@zju.edu.cn

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Abstract

BACKGROUND

Many essential oils were reported to be used as natural, environmental friendly antimicrobial agents. The antifungal activity in vitro and in vivo of an essential oil extracted from Chinese local Laurus nobilis leaves against Alternaria alternata has been studied.

RESULT

The main components of the essential oil were investigated by means of gas chromatography–mass spectrometry and 14 components were identified. The main ones were eugenol, caryophyllene and cinnamaldehyde. The antifungal test showed that at 800 µg mL−1 of L. nobilis oil completely inhibited the growth of A. alternata. In addition, the conidial germination of the pathogen was significantly inhibited at 200 µg mL−1, and the weight of mycelia efficiently decreased at 500 µg mL−1. The in vivo assay indicated that 500 µg mL−1 L. nobilis oil was effective in protecting cherry tomatoes (Lycopersicon esculentum) from infection of A. alternata, with an inhibition ratio of 33.9%. Scanning electron microscopy observations of the pathogen revealed significant morphological alterations in the hyphae.

CONCLUSION

This work suggested that L. nobilis oil could be used as a potential fungicide to control the post-harvest disease caused by A. alternata. © 2013 Society of Chemical Industry

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