Porcine plasma protein (PPP) showed inhibitory activity towards trypsin, papain, digestive enzymes and modori-inducing proteinases from bigeye snapper. Among the fractions prepared, fraction IV-1 exhibited the highest inhibitory activity against all proteinases tested and the autolysis of fish muscle. At a level of 0.5% (w/w), both PPP and fraction IV-1 effectively prevented the degradation of myosin heavy chain in fish muscle. The inhibitory activity of fraction IV-1 was stable up to 60 °C. Incorporation of fraction IV-1 significantly increased the breaking force, deformation and water-holding capacity of surimi gel from bigeye snapper (P < 0.05). However, no increase in breaking force and deformation was found when fraction IV-1 was added at a level above 0.3% (w/w) (P > 0.05). No significant change in whiteness of surimi gel was observed with the addition of fraction IV-1 (P > 0.05).
© 2001 Society of Chemical Industry