Nuclear localization of annexin A1 is a prognostic factor in oral squamous cell carcinoma

Authors

  • Chiao-Ying Lin DDS,

    1. School of Dentistry and Graduate Institute of Clinical Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan
    2. Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan
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  • Yung-Ming Jeng MD,

    1. Graduate Institute of Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan
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  • Han-Yi Chou PhD,

    1. Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan
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  • Hey-Chi Hsu DDS,

    1. Graduate Institute of Pathology, College of Medicine, National Taiwan University, Taipei, Taiwan
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  • Ray-Hwang Yuan MD, PhD,

    1. Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
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  • Chun-Pin Chiang DDS, PhD,

    1. School of Dentistry and Graduate Institute of Clinical Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan
    2. Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan
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  • Mark Yen-Ping Kuo DDS, PhD

    Corresponding author
    1. School of Dentistry and Graduate Institute of Clinical Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan
    2. Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan
    • School of Dentistry, College of Medicine, National Taiwan University, 7 Chung-Shan South Road, Taipei 10016, Taiwan; Fax: +886-223820785.
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Abstract

Background and Objectives

To investigate whether annexin A1 (ANXA1) expression is a marker in predicting the prognosis of oral cancer patients.

Methods

We immunohistochemically examined the expression of ANXA1 in 66 cases of oral epithelial dysplasia (OED) and 115 cases of oral squamous cell carcinoma (OSCC). The results were correlated with the clinicopathological parameters of tumors and overall patient survival.

Results

In normal oral mucosa, ANXA1 staining was predominantly located on the cell membrane. In OED and OSCC specimens, membranous staining decreased, whereas nuclear staining increased. Positive nuclear staining was observed in 9 of 66 (13.64%) OED cases and 63 of 115 (54.8%) OSCCs. Kaplan–Meier curves showed that OSCC patients with ANXA1 nuclear staining had significantly shorter overall lengths of survival (P = 0.00036 by the log-rank test). Multivariate analysis showed that ANXA1 nuclear staining is a significant predictor of poor overall survival. And oral cancer SAS cells treated with hepatocyte growth factor (HGF) can induce ANXA1 protein translocation from cytoplasm to nucleus. Cells pretreated with LY294002 (PI3K inhibitor) almost completely inhibited (88.3% inhibition) HGF-mediated ANXA1 nuclear translocation.

Conclusions

The nuclear localization of ANXA1 protein is a frequent event and could be used as a prognostic factor in OSCC. J. Surg. Oncol. 2008;97:544–550. © 2008 Wiley-Liss, Inc.

Ancillary