Correlations of the expression of vascular endothelial growth factor B and its isoforms in hepatocellular carcinoma with clinico-pathological parameters
Article first published online: 6 JUN 2008
Copyright © 2008 Wiley-Liss, Inc.
Journal of Surgical Oncology
Volume 98, Issue 3, pages 190–196, 1 September 2008
How to Cite
Kanda, M., Nomoto, S., Nishikawa, Y., Sugimoto, H., Kanazumi, N., Takeda, S. and Nakao, A. (2008), Correlations of the expression of vascular endothelial growth factor B and its isoforms in hepatocellular carcinoma with clinico-pathological parameters. J. Surg. Oncol., 98: 190–196. doi: 10.1002/jso.21095
- Issue published online: 14 AUG 2008
- Article first published online: 6 JUN 2008
- Manuscript Accepted: 5 MAY 2008
- Manuscript Received: 22 JAN 2008
- hepatocellular carcinoma;
The vascular endothelial growth factor (VEGF) is involved in the growth of cancer cells through angiogenesis. At present the role of VEGF-B has not been clarified completely. We investigated correlations of the expression of VEGF-B and its isoforms, VEGF-B167 and VEGF-B186, by alternative splicing in hepatocellular carcinoma (HCC) with the pathological findings and prognosis.
Forty-eight patients with HCC were investigated. We examined the mRNA expression of total VEGF-B, VEGF-B167 and VEGF-B186 in primary HCC and non-cancerous tissues using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) analysis.
In 16 (33.3%) of 48 HCCs, the expression of total VEGF-B increased compared with the corresponding non-cancerous liver tissues. Regarding the isoforms, the expression of VEGF-B167 and VEGF-B186 was increased in 17 (35.4%) of 48 and 33 (68.75%) of 48 HCCs, respectively. Cases with high expression level of total VEGF-B in HCC significantly correlated with the advanced pathological stage (P < 0.018), tumor multiplicity (P < 0.033), vascular invasion (P < 0.045) and lack of capsule formation (P < 0.027). The result in VEGF-B167 was similar to total VEGF-B.
Our results indicated that the expression of VEGF-B is correlated with tumor growth and invasiveness in HCC. VEGF-B167 seemed to be the clinically dominant isoform. J. Surg. Oncol. 2008;98:190–196. © 2008 Wiley-Liss, Inc.