Masaya Suzuki, Shinji Kageyama and Kazuya Shinmura contributed equally to this work.
Inverse relationship between the length of the EGFR CA repeat polymorphism in lung carcinoma and protein expression of EGFR in the carcinoma†
Article first published online: 5 AUG 2008
Copyright © 2008 Wiley-Liss, Inc.
Journal of Surgical Oncology
Volume 98, Issue 6, pages 457–461, 1 November 2008
How to Cite
Suzuki, M., Kageyama, S., Shinmura, K., Okudela, K., Bunai, T., Nagura, K., Igarashi, H., Kiyose, S., Mori, H., Tao, H., Goto, M., Takamochi, K., Mochizuki, T., Suzuki, K., Ohashi, R., Ogawa, H., Yamada, T., Niwa, H., Tsuneyoshi, T. and Sugimura, H. (2008), Inverse relationship between the length of the EGFR CA repeat polymorphism in lung carcinoma and protein expression of EGFR in the carcinoma. J. Surg. Oncol., 98: 457–461. doi: 10.1002/jso.21130
- Issue published online: 24 OCT 2008
- Article first published online: 5 AUG 2008
- Manuscript Accepted: 8 JUL 2008
- Manuscript Received: 30 JAN 2008
- Ministry of Health, Labour and Welfare. Grant Number: 15-22 19-19
- Ministry of Education, Culture, Sports, Science and Technology of Japan for Priority Areas. Grant Number: 18014009
- Japan Society for the Promotion of Science. Grant Numbers: 19790286, 17790944
- 21st Century COE Program ‘Medical Photonics’
- Smoking Research Foundation
- gene copy number;
- immunohistochemical analysis
Background and Objectives
There is a CA dinucleotide repeat polymorphism in the first intron of the epidermal growth factor receptor (EGFR) gene. Our aim was to examine the relationship between the CA repeat length in lung carcinoma and EGFR mutation, EGFR gene copy number, and EGFR protein expression level in the carcinoma.
We examined 168 lung carcinomas for the length of the CA repeat polymorphism by PCR–polyacrylamide gel electrophoresis analysis, for EGFR mutations by sequencing analysis, for EGFR gene copy number by fluorescence in situ hybridization analysis, and for EGFR protein expression by immunohistochemical analysis.
When the carcinomas were divided into two groups according to the length of their CA repeat polymorphism, the EGFR protein expression level was found to be significantly higher in the shorter allele group than in the longer allele group (P = 0.0116), but its length was not associated with EGFR somatic mutations, high EGFR gene copy numbers, or clinicopathological factors, such as histological type or stage.
The results suggested that the length of the EGFR CA repeat polymorphism in lung carcinoma is inversely related with level of EGFR protein expression in the carcinoma. J. Surg. Oncol. © 2008 Wiley-Liss, Inc.