None of the authors have competing interests to declare.
Article first published online: 11 JUN 2010
Copyright © 2010 Wiley-Liss, Inc.
Journal of Surgical Oncology
Volume 102, Issue 5, pages 482–489, 1 October 2010
How to Cite
Zhao, G., Cui, J., Qin, Q., Zhang, J., Liu, L., Deng, S., Wu, C., Yang, M., Li, S. and Wang, C. (2010), Mechanical stiffness of liver tissues in relation to integrin β1 expression may influence the development of hepatic cirrhosis and hepatocellular carcinoma. J. Surg. Oncol., 102: 482–489. doi: 10.1002/jso.21613
Gang Zhao and Jing Cui contributed equally to this work.
- Issue published online: 11 JUN 2010
- Article first published online: 11 JUN 2010
- Manuscript Accepted: 13 APR 2010
- Manuscript Received: 7 NOV 2009
- National Natural Science Foundation of China. Grant Number: 30600594
- hepatocellular carcinoma;
- hepatic cirrhosis;
- mechanical stiffness;
- integrin β1;
- extracellular matrix;
- atomic force microscopy
Background and Objectives
Increased rigidity of the extracellular matrix (ECM) is commonly associated with hepatocellular carcinoma (HCC). The purpose of this study was to quantitate the mechanical stiffness of various hepatic tissues, evaluate integrin β1 expression, and investigate the correlation between these two factors in the development of HCC.
Twenty-three normal specimens, 152 cases of cirrhosis, and 105 cases of HCC were included in this study. The mechanical stiffness of the ECM of each specimen was detected using atomic force microscopy to calculate elastic modulus (E) values. Integrin β1 expression was also evaluated using semi-quantitative RT-PCR, western blot, and immunohistochemistry. Expression of integrin β1 in HepG2 cells plated on substrates with different mechanical stiffnesses was also evaluated.
A positive correlation between ECM mechanical stiffness and integrin β1 expression was detected. Expression of integrin β1 also correlated with Edmondson pathologic grade, encapsulation, metastasis, and HBV infection (P < 0.01). In vitro, expression of integrin β1 by HepG2 cells was also significantly higher when the cells were plated on stiffer substrates.
Expression of integrin β1 is regulated by the mechanical stiffness of the ECM, and correlates with the invasion and metastasis events of HCC in patients with cirrhosis. J. Surg. Oncol. 2010;102:482–489. © 2010 Wiley-Liss, Inc.