Cluster analysis of claudin-1 and -4, E-cadherin, and β-catenin expression in colorectal cancers
Article first published online: 24 FEB 2011
Copyright © 2011 Wiley-Liss, Inc.
Journal of Surgical Oncology
Volume 103, Issue 7, pages 674–686, 1 June 2011
How to Cite
Matsuoka, T., Mitomi, H., Fukui, N., Kanazawa, H., Saito, T., Hayashi, T. and Yao, T. (2011), Cluster analysis of claudin-1 and -4, E-cadherin, and β-catenin expression in colorectal cancers. J. Surg. Oncol., 103: 674–686. doi: 10.1002/jso.21854
- Issue published online: 26 APR 2011
- Article first published online: 24 FEB 2011
- Manuscript Accepted: 8 DEC 2010
- Manuscript Received: 4 AUG 2010
- Japan Society for the Promotion of Science. Grant Number: 21590394
- cluster analysis;
- colon carcinoma
Background and Objectives
Intercellular adhesion mediated by the claudin and cadherin/catenin complex is a prerequisite for epithelial integrity and differentiation, and has been suggested to be frequently disturbed in cancers. The aim of this study was to assess the relationship between such abnormality and clinicopathological features of colorectal carcinomas.
Immunohistochemical analysis of claudin-1 and -4, E-cadherin, and β-catenin was performed on a series of 156 cases.
Significant positive associations (P < 0.05–0.001) were found with immunoreactivity for each except nuclear β-catenin. Reduced expression was correlated with poor tumor differentiation (claudin-1, P = 0.035; claudin-4, P = 0.011; E-cadherin, P < 0.001; membranous β-catenin, P = 0.002), an advanced TNM stage (claudin-1, P = 0.002; claudin-4, P = 0.008), and a poor prognosis. On multivariate analysis, reduced expression of E-cadherin (P < 0.001) and β-catenin (P = 0.048) at invasive fronts proved to be an independent predictor of short survival. Hierarchical cluster analysis identified three distinct groups with a good, intermediate, and poor prognosis, having an independent survival outcome by multivariate analysis (good or intermediate vs. poor: hazard ratio, 2.66; 95% confidence interval, 1.54–4.60; P < 0.001).
Disruption of cell adhesion molecules correlates with tumor differentiation and progression in colorectal carcinomas. Specific marker profiles were identified here as independent prognostic indicators. J. Surg. Oncol. 2011;103:674–686. © 2011 Wiley-Liss, Inc.