Effects of E-cadherin (CDH1) gene promoter polymorphisms on the risk and clinicopathological development of hepatocellular carcinoma

Authors

  • Ming-Hsien Chien PhD,

    1. Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan
    2. Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
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  • Kun-Tu Yeh MD,

    1. Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan
    2. School of Medicine, Chung Shan Medical University, Taichung, Taiwan
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  • Yi-Ching Li PhD,

    1. Department of Pharmacology, Chung Shan Medical University, Taichung, Taiwan
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  • Yi-Hsien Hsieh PhD,

    1. Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan
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  • Chien-Huang Lin PhD,

    1. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan
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  • Meng-Shih Weng PhD,

    1. Department of Nutritional Science, Fu-Jen Catholic University, Taipei, Taiwan
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  • Wu-Hsien Kuo MD, PhD,

    Corresponding author
    1. Department of Medicine, Armed-Force Taichung General Hospital, Taichung, Taiwan
    2. General Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan
    • Institute of Medicine, Chung Shan Medical University, 110 Chien-Kuo N. Road, Section 1, Taichung, Taiwan. Fax: +886–4-24723229.
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  • Shun-Fa Yang PhD

    Corresponding author
    1. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
    2. Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan
    • Institute of Medicine, Chung Shan Medical University, 110 Chien-Kuo N. Road, Section 1, Taichung, Taiwan. Fax: +886–4-24723229.
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Abstract

Background and Objectives

Hepatocellular carcinoma (HCC) is one of the most frequent malignant neoplasms worldwide, and is the second leading cause of cancer death in Taiwan. E-cadherin is an epithelial cell adhesion molecule, and decreased E-cadherin expression in HCC is associated with a poor prognosis. This study investigates the effects of single nucleotide polymorphisms (SNPs) in the E-cadherin/CDH1 gene promoter on the risk and clinicopathological characteristics of HCC

Methods

131 HCC patients and 347 controls were recruited for this study. Genetic polymorphisms of CDH1-160 and -347 were analyzed by PCR-RFLP genotyping analysis.

Results

After adjusting for other confounders, results show that individuals with the CDH1-347G/GA or GA/GA polymorphic genotypes had a significantly higher risk of developing HCC than those with the wild-type (G/G) genotype (adjusted odds ratio = 2.477; 95%CI: 1.421–4.319). Furthermore, patients with HCC with at least one mutant A allele of CDH1-160 had a 4.031-fold risk of progressing to stage III or IV.

Conclusions

This study shows that SNPs in CDH1-347 gene are associated with an increased risk of HCC, and at least one mutant A allele of CDH1-160 gene is associated with the development of stage III or IV of HCC in Taiwanese. J. Surg. Oncol. 2011; 104:299–304. © 2011 Wiley-Liss, Inc.

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