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Expression of microRNA-218 in human pancreatic ductal adenocarcinoma and its correlation with tumor progression and patient survival

Authors

  • Ziman Zhu MD,

    1. Department of Hepatobiliary Surgery, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Yuefang Xu MM,

    1. Department of Pharmacy, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Jundong Du MM,

    1. Department of Hepatobiliary Surgery, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Jingwang Tan MD,

    1. Department of Hepatobiliary Surgery, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, People's Republic of China
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  • Dr. Huabao Jiao MD

    Corresponding author
    1. Department of Hepatobiliary Surgery, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing, People's Republic of China
    • Correspondence to: Dr. Hua-Bao Jiao, Department of Hepatobiliary Surgery, First Hospital Affiliated to the Chinese PLA General Hospital, Fucheng Road 51, Haidian District, Beijing 100048, People's Republic of China. Fax: +86-10-66848633.

      E-mail: jiaohuabao@yeah.net

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  • Conflict of interest: none.
  • Ziman Zhu, Yuefang Xu and Jundong Du contributed equally to this work and should be considered as co-first authors.

Abstract

Background

Our aim was to analyze clinicopathologic and prognostic values of microRNA (miR)-218 in pancreatic ductal adenocarcinima (PDAC).

Methods

TaqMan quantitative RT-PCR was used to determine the expression of miR-218 in human PDAC cells and tissue samples. The association of miR-218 expression with clinicopathologic variables was analyzed. Kaplan–Meier survival analysis was performed to analyze the association of miR-218 expression with recurrence-free survival or overall survival of patients. Univariate and multivariate Cox regression analyses were performed.

Results

The relative level of miR-218 in PDAC cells was significantly lower than that in normal human pancreatic duct epithelial cell line. Also, the mean level of miR-218 in PDAC tissues was significantly lower than that in normal pancreatic tissues. Statistical analyses indicated that low miR-218 expression was closely associated with poor tumor differentiation, advanced tumor stage, higher incidence of lymph node metastasis, and tumor recurrence. Kaplan–Meier survival analyses showed that patients with low miR-218 expression had lower recurrence-free and overall survival than those with high miR-218 expression. Univariate and multivariate Cox regression analyses showed that miR-218 might be an independent prognostic factor.

Conclusion

Reduced miR-218 in PDAC tissues was correlated with tumor progression, and might be an independent poor prognostic factor for patients. J. Surg. Oncol. 2014 109:89–94. © 2013 Wiley Periodicals, Inc.

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