HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure

Authors

  • Laura Mercolini,

    1. Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum – University of Bologna, Bologna, Italy. Fax: +39-051-2099740
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  • Maria Addolorata Saracino,

    1. Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum – University of Bologna, Bologna, Italy. Fax: +39-051-2099740
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  • Francesca Bugamelli,

    1. Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum – University of Bologna, Bologna, Italy. Fax: +39-051-2099740
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  • Anna Ferranti,

    1. Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum – University of Bologna, Bologna, Italy. Fax: +39-051-2099740
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  • Marco Malaguti,

    1. Department of Biochemistry “G. Moruzzi”, Faculty of Pharmacy, Alma Mater Studiorum – University of Bologna, Bologna, Italy
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  • Silvana Hrelia,

    1. Department of Biochemistry “G. Moruzzi”, Faculty of Pharmacy, Alma Mater Studiorum – University of Bologna, Bologna, Italy
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  • Maria Augusta Raggi

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Abstract

An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm×4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03–5.00 ng/mL for MLT, 3–500 ng/mL for t-RSV and 1–150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.

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